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Updated: May 18, 2026

A Mouse Model of Vascularized Heterotopic Spleen Transplantation for Studying Spleen Cell Biology and Transplant Immunity
08:04

A Mouse Model of Vascularized Heterotopic Spleen Transplantation for Studying Spleen Cell Biology and Transplant Immunity

Published on: June 11, 2019

The mouse lymph node as an ectopic transplantation site for multiple tissues.

Junji Komori1, Lindsey Boone, Aaron DeWard

  • 1McGowan Institute for Regenerative Medicine, Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA.

Nature Biotechnology
|September 25, 2012
PubMed
Summary

Mouse lymph nodes can support the growth of transplanted cells, enabling ectopic organ formation. This cell therapy approach successfully treated metabolic disease, restored immunity, and normalized glucose control in mice.

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Area of Science:

  • Regenerative Medicine
  • Immunology
  • Transplantation Biology

Background:

  • Cell-based therapy offers an alternative to organ transplantation for tissue repair.
  • Current cell transplantation methods for organ repair face limitations.
  • The potential of lymph nodes as sites for cell engraftment and organogenesis is underexplored.

Purpose of the Study:

  • To investigate the feasibility of using mouse lymph nodes for cell engraftment and ectopic organ formation.
  • To evaluate the therapeutic potential of lymph node-targeted cell transplantation for various diseases.
  • To establish lymph nodes as a practical site for regenerative medicine strategies.

Main Methods:

  • Direct injection of hepatocytes, thymuses, and pancreatic islets into mouse lymph nodes.

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A Heterotopic Mouse Model for Studying Laryngeal Transplantation
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Last Updated: May 18, 2026

A Mouse Model of Vascularized Heterotopic Spleen Transplantation for Studying Spleen Cell Biology and Transplant Immunity
08:04

A Mouse Model of Vascularized Heterotopic Spleen Transplantation for Studying Spleen Cell Biology and Transplant Immunity

Published on: June 11, 2019

A Heterotopic Mouse Model for Studying Laryngeal Transplantation
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A Heterotopic Mouse Model for Studying Laryngeal Transplantation

Published on: January 13, 2023

  • Assessment of ectopic liver mass formation and its impact on metabolic disease.
  • Evaluation of immune system reconstitution and graft rejection capabilities in transplanted mice.
  • Monitoring of glucose control in diabetic mice following pancreatic islet lymph node transplantation.

    • Main Results:

    • Engraftment and growth of healthy cells from multiple tissues within mouse lymph nodes were demonstrated.
    • Hepatocyte transplantation into lymph nodes generated sufficient ectopic liver mass to rescue mice with lethal metabolic disease.
    • Transplanted thymuses in lymph nodes restored functional immune systems in athymic nude mice, enabling graft rejection.
    • Pancreatic islets injected into lymph nodes of diabetic mice successfully restored normal glucose regulation.

    Conclusions:

    • Mouse lymph nodes can serve as a supportive environment for cell engraftment and the development of functional ectopic organs.
    • Targeting lymph nodes for cell transplantation presents a practical and effective strategy for restoring, maintaining, or improving organ function.
    • This approach holds significant promise for regenerative medicine, offering an alternative to traditional organ transplantation.