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Related Experiment Video

Updated: May 18, 2026

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications
13:14

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

Submicrometre geometrically encoded fluorescent barcodes self-assembled from DNA.

Chenxiang Lin1, Ralf Jungmann, Andrew M Leifer

  • 1Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts 02115, USA.

Nature Chemistry
|September 25, 2012
PubMed
Summary

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DNA origami nanorods create unique fluorescent barcodes for molecular identification. This advance overcomes limitations in fluorescence microscopy, enabling precise imaging of biomolecules in their natural state.

Area of Science:

  • Biotechnology
  • Molecular Imaging
  • Nanotechnology

Background:

  • Distinguishing numerous molecular species with high spatiotemporal resolution is crucial in biomedical research.
  • Current fluorescence microscopy multiplexing is limited by the number of spectrally distinct fluorophores.

Purpose of the Study:

  • To develop a novel method for creating highly multiplexed fluorescent barcodes using DNA origami.
  • To enhance the capability of fluorescence microscopy for identifying diverse molecular entities.

Main Methods:

  • Utilized (deoxy)ribonucleic acid (DNA) origami technology to fabricate submicrometre nanorods.
  • Engineered spatial control over fluorophore positioning on DNA nanorods to generate distinct fluorescent barcodes.
  • Employed epifluorescence and total internal reflection fluorescence microscopy for barcode decoding.

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Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray
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Related Experiment Videos

Last Updated: May 18, 2026

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications
13:14

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
10:00

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray
09:05

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Published on: January 6, 2016

Main Results:

  • Successfully generated 216 unique fluorescent barcodes with unambiguous decoding.
  • Demonstrated super-resolution barcodes with feature spacing of approximately 40 nm for higher spatial information density.
  • Successfully tagged yeast surface receptors with DNA nanorod barcodes.

Conclusions:

  • DNA origami nanorods serve as effective fluorescent barcodes, significantly expanding multiplexing capabilities in microscopy.
  • The developed barcodes show potential as in situ imaging probes for various biomolecular and cellular targets in native environments.