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Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment

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Published on: November 17, 2018

Murine gut microbiota and transcriptome are diet dependent.

Erica M Carlisle¹, Valeriy Poroyko, Michael S Caplan

¹Department of Surgery, The University of Chicago Medical Center, Chicago, IL 60637, USA.

Annals of Surgery

|September 25, 2012

Summary

This summary is machine-generated.

Formula feeding alters the gut microbiota and host gene expression in mice. This shift impacts gut integrity and immune function, potentially increasing disease vulnerability in formula-fed infants.

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Area of Science:

Microbiology
Genomics
Immunology

Background:

Formula-fed infants face increased risks for diseases like necrotizing enterocolitis.
Understanding the interplay between diet, gut microbes, and host immunity is crucial.

Purpose of the Study:

To investigate the impact of formula feeding on gut microbiota composition.
To analyze host gene expression changes in response to formula feeding.
To compare formula-fed (FF) and maternally-fed (MF) mice.

Main Methods:

Colonic tissue collected after 72 hours of FF or MF.
16S rRNA gene sequencing for microbial analysis.
RNA sequencing (Solexa) for host transcriptome profiling.
Bioinformatic analysis including BLAST and KOG classification.

Main Results:

Formula feeding altered dominant bacterial phyla (Proteobacteria, Bacteroidetes) and genera (Serratia, Lactococcus) compared to maternal feeding.
Significant changes in host gene expression (148 genes) were observed, including increased oxidative stress marker Heme oxygenase 1.
Decreased vinculin suggests impaired gut structural integrity in formula-fed mice.

Conclusions:

Formula feeding significantly shifts gut microbiota composition and host gene expression.
These alterations affect gut structural integrity, oxidative stress, and immune function.
Investigating these changes offers insights into diet-related disease pathogenesis in neonates.