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Updated: May 18, 2026

An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis
Published on: September 15, 2015
Identification and distribution of high-abundance proteins in the octopus spring microbial mat community
Courtney S Schaffert1, Christian G Klatt, David M Ward
1Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska, USA.
Abstract:
A shotgun metaproteomics approach was employed to identify proteins in a hot spring microbial mat community. We identified 202 proteins encompassing 19 known functions from 12 known phyla. Importantly, we identified two key enzymes involved in the 3-hydroxypropionate CO(2) fixation pathway in uncultivated Roseiflexus spp., which are known photoheterotrophs.
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