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Updated: May 18, 2026
Optimization of Antigen Preparation for Hemagglutination Inhibition Assay of Newcastle Disease Virus Serology
Published on: January 24, 2025
[Gene optimization, protein expression, purification and characterization of the HDV antigen for diagnosis]
Jun-Ying Ding1, Min-Zhuo Guo, Yao Yi
1National Institute for Viral Disease Control and Prevention, Beijng 102206, China. jyd0311@126.com
Objective:
To prepare HDAg with biological activities as a candidate of diagnostic reagent.
Methods:
To synthesize HDV gene fragment after codon optimization. To construct a thio-fused recombinant plasmid based on M48 expression vector. To express in E. coli induced by IPTG. To purify the protein by affinity chromatography followed by characterization in ELISA:
Results:
Plasmid construction was verified by enzyme digestion. SDS-PAGE indicated the molecular weight of the protein was the same as we expectation. ELISA proved its affinity with HDV antibodies.
Conclusion:
HDAg was obtained successfully and it will pave the road to the research of HDV diagnostic reagent.
