Related Experiment Video
Updated: May 18, 2026
Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle
Published on: February 1, 2017
[Preparation and identification of a recombinant hepatitis C virus (HCV) based on sindbis virus vector]
Juan-Juan Fu1, Wu-Yang Zhu, Jiang-Jiao Li
1Kunming Medical College, Kunming 650500, China.
Objective:
To construct the recombinant virus-like particles containing HCV envelope glycoprotein E1E2 based on sindbis virus vector.
Methods:
The gene encoding HCV envelope glycoprotein E1E2 was cloned into sindbis virus vector to construct recombinant plasmids pBR-XJE1E2 and pVA-XJE1E2, and transfect them into BHK-21 cells to obtain recombinant virus-like particles. The expression of E1 and E2 protein were verified by Western Blot and indirect immunofluorescent assay (IFA).
Results:
The results of restriction enzyme digestion, PCR and sequencing analysis showed that the recombinant plasmids were constructed successfully. And the results of RT-PCR, Western blotting and IFA detection showed that the transfect cells could package HCV-like particles of expressing structural proteins E1E2.
Conclusion:
The recombinant expression plasmids pBR-XJE1E2 and pVA-XJE1E2 based on sindbis virus vector could package HCV-like particles in eukaryotic cell, which provides a foundation for further study of its in vivo animal immune response.
