Related Experiment Video
Updated: May 18, 2026
Measuring Influenza Neuraminidase Inhibition Antibody Titers by Enzyme-linked Lectin Assay
Published on: September 6, 2016
[Primary establishment of an alphaLISA assay for detection of HAV IgM]
Objective:
To develop an AlphaLISA method for detection of antibody of Hepatitis A virus.
Methods:
After hepatitis A virus antigen was concentrated and biotinylated, optimal biotinylated HAV antigen, donor bead, acceptor bead concentration have been explored and determined by both dot-blotting and AlphaLISA methods. 97 samples including 23 serums from patients HAV infected and 70 serums from blood donors have been detected by new AlphaLISA method.
Results:
The sensitivity and specificity for anti-HAV IgM were 78% and 98.5%, the positive and negative predictive value were 95% and 93%.
Conclusion:
A homogeneous and fast AlphaLISA assay for detection of HAV IgM has been established by preliminary verification on samples.
