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Related Experiment Video

Updated: May 18, 2026

Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone
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Published on: April 11, 2013

Full-field near-field optical microscope for cell imaging.

Thomas Barroca1, Karla Balaa, Sandrine Lévêque-Fort

  • 1Centre d'Imageries Plasmoniques Appliquées, Institut Langevin, Ecole Supérieure de Physique et de Chimie Industrielles (ESPCI) ParisTech, CNRS UMR 7587, Université Paris Diderot, Paris, France.

Physical Review Letters
|September 26, 2012
PubMed
Summary

We developed a new fluorescence microscopy technique using supercritical near-field emission to image live cell membranes. This method enhances image sensitivity and axial confinement without compromising lateral resolution.

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Area of Science:

  • Biophysics
  • Optical Microscopy
  • Cell Biology

Background:

  • Live cell membrane imaging is crucial for understanding cellular processes.
  • Conventional fluorescence microscopy faces limitations in resolution and sensitivity.
  • Supercritical near-field emission offers potential for enhanced optical imaging.

Purpose of the Study:

  • To introduce a novel full-field fluorescence microscopy method for live cell membrane imaging.
  • To leverage supercritical near-field emission for improved imaging performance.
  • To achieve high axial confinement and enhanced sensitivity without lateral resolution loss.

Main Methods:

  • Developed a technique based on supercritical near-field emission.
  • Utilized image subtraction to extract self-interference between undercritical and supercritical light.
  • Implemented a virtual mask in the objective back focal plane to block subcritical emission.

Main Results:

  • Achieved 100 nm axial confinement.
  • Enhanced image sensitivity significantly.
  • Maintained lateral resolution without damage.
  • Demonstrated simultaneous imaging of inner cell parts and membranes.
  • The technique is easy to implement with standard illumination.

Conclusions:

  • The new microscopy method provides superior axial confinement and sensitivity for live cell membrane imaging.
  • The virtual mask approach offers advantages over physical masks in optical microscopy.
  • This technique is a valuable tool for live cell imaging and biological research.