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Related Concept Videos

Pinching-off of Coated Vesicles01:32

Pinching-off of Coated Vesicles

Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...

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Updated: May 18, 2026

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
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3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

Published on: October 1, 2014

Three-dimensional numerical simulation of vesicle dynamics using a front-tracking method.

Alireza Yazdani1, Prosenjit Bagchi

  • 1Department of Mechanical & Aerospace Engineering, Rutgers University, the State University of New Jersey, Piscataway, 08854, USA.

Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics
|September 26, 2012
PubMed
Summary
This summary is machine-generated.

Vesicle dynamics in shear flow are complex, not self-similar as previously thought. Three parameters, not two, govern behavior, with deviations from theory even without noise.

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Area of Science:

  • Biophysics
  • Fluid Dynamics
  • Computational Science

Background:

  • Vesicle dynamics in shear flow are crucial for understanding cellular mechanics.
  • Existing theoretical models offer limited applicability to complex vesicle behaviors.

Purpose of the Study:

  • To investigate the parametric dependence and self-similarity of vesicle dynamics in linear shear flow.
  • To quantify vesicle deformation and analyze shape dynamics through numerical simulations.
  • To compare simulation results with theoretical models and experimental data.

Main Methods:

  • Three-dimensional numerical simulation utilizing the front-tracking method.
  • Detailed comparison of simulation outcomes against established theoretical frameworks and experimental findings.
  • Analysis of vesicle shape dynamics, including deformation and spectral analysis.

Main Results:

  • Vesicle dynamics are governed by excess area, viscosity ratio, and shear rate, deviating from proposed two-parameter self-similar models.
  • Linear scaling of tank-treading angle is limited to nearly spherical vesicles; breakdown occurs at higher excess areas.
  • Vesicle deformation saturates at high shear rates, matching theoretical predictions for near-spherical shapes.
  • Unsteady dynamics exhibit vacillating-breathing and trembling motions, with the latter showing significant 3D deformation.

Conclusions:

  • Theoretical models show limited applicability, necessitating refined understanding of vesicle dynamics.
  • Vesicle dynamics are more complex than previously described by self-similar models, influenced by multiple parameters.
  • Numerical simulations provide crucial insights into complex behaviors like trembling motion and harmonic content of vesicle shapes.