Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

p97 Inhibition Synergistically Enhances Hypomethylating Therapy through Targeting of PLK1 in Acute Myeloid Leukemia.

Cancer research communications·2026
Same author

DeepDeconUQ estimates malignant cell fraction prediction intervals in bulk RNA-seq tissue.

PLoS computational biology·2025
Same author

Enhance the therapeutic efficacy of human umbilical cord-derived mesenchymal stem cells in prevention of acute graft-versus-host disease through CRISPLD2 modulation.

Stem cell research & therapy·2025
Same author

A 6-tsRNA signature for early detection, treatment response monitoring, and prognosis prediction in diffuse large B cell lymphoma.

Blood cancer journal·2025
Same author

A novel replicase-mediated self-amplifying RNA amplification mechanism of the SARS-CoV-2 replication-transcription system.

Biochemical and biophysical research communications·2025
Same author

Exploring treatment-driven subclonal evolution of prognostic triple biomarkers: Dual gene fusions and chimeric RNA variants in novel subtypes of acute myeloid leukemia patients with KMT2A rearrangement.

Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy·2025

Related Experiment Video

Updated: May 18, 2026

Isolation of microRNAs from Tick Ex Vivo Salivary Gland Cultures and Extracellular Vesicles
08:03

Isolation of microRNAs from Tick Ex Vivo Salivary Gland Cultures and Extracellular Vesicles

Published on: April 6, 2022

Identify intronic microRNA with bioinformatics.

Chih-Hao Kuo1, Mark D Goldberg, Shi-Lung Lin

  • 1Department of Cell and Neurobiology, University of Southern California, Los Angeles, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|September 26, 2012
PubMed
Summary
This summary is machine-generated.

This study introduces a rapid bioinformatics method to discover microRNAs (miRNAs) within gene introns. This approach aids in understanding gene regulation and miRNA

More Related Videos

mirMachine: A One-Stop Shop for Plant miRNA Annotation
06:16

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

Related Experiment Videos

Last Updated: May 18, 2026

Isolation of microRNAs from Tick Ex Vivo Salivary Gland Cultures and Extracellular Vesicles
08:03

Isolation of microRNAs from Tick Ex Vivo Salivary Gland Cultures and Extracellular Vesicles

Published on: April 6, 2022

mirMachine: A One-Stop Shop for Plant miRNA Annotation
06:16

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • MicroRNAs (miRNAs) are crucial noncoding RNA regulators of gene expression.
  • Identifying miRNAs experimentally is challenging due to their small size and specific expression patterns.
  • Existing bioinformatics methods often rely on complete genomic sequences.

Purpose of the Study:

  • To develop a rapid bioinformatics approach for identifying novel microRNAs (miRNAs).
  • To specifically mine for miRNAs located within gene introns.
  • To provide intron locations for identified potential miRNAs to facilitate gene interaction studies.

Main Methods:

  • A novel bioinformatics strategy was employed to search for miRNA sequences within gene introns.
  • The approach focuses on mining gene-specific introns rather than complete genomes.
  • The method identifies potential miRNA sequences and their precise intronic locations.

Main Results:

  • A rapid bioinformatics approach for mining microRNAs (miRNAs) from gene introns was successfully developed.
  • The method efficiently identifies potential miRNA sequences and their intronic locations.
  • This facilitates the study of intronic miRNAs and their regulatory roles.

Conclusions:

  • The developed bioinformatics approach offers a fast and effective way to discover intronic microRNAs (miRNAs).
  • Identifying intronic miRNAs and their locations is vital for understanding gene regulation during development.
  • This method supports research into gene-gene interactions mediated by miRNAs.