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Updated: May 18, 2026

mirMachine: A One-Stop Shop for Plant miRNA Annotation
Published on: May 1, 2021
Enrichment analysis of miRNA targets
1College of Bioengineering, Henan University of Technology, Zhengzhou, China. xujz0451@gmail.com
Abstract:
MicroRNAs (miRNAs) are a class of ∼22-nucleotide endogenous noncoding RNAs which regulate target gene expression via repressing translation or promoting mRNAs degradation. Any individual mammalian miRNA often has more than a hundred predicted mRNA targets and that close to one thirds of all mRNA transcripts bear one or more conserved miRNA binding sites in their 3'-untranslated region. Enrichment analysis of miRNA targets has become a standard technique to elucidating hierarchical functions of miRNAs in gene regulatory networks. In this protocol, we discuss analytical methods and use of computational tools in a step-by-step manner. Important details are also provided to help researchers choose more appropriate tools for a given type of analysis. Available Web-based resources for enrichment analysis of miRNA targets are summarized.
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