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Neurogenesis Using P19 Embryonal Carcinoma Cells

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MicroRNA expression profiling during neural differentiation of mouse embryonic carcinoma P19 cells

Botao Zhao1, Bing Huang, Wei Li

  • 1School of Life Sciences, Shanghai University, Shanghai, China.

Methods in Molecular Biology (Clifton, N.J.)
|September 26, 2012
PubMed

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MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...