Related Experiment Video
Updated: May 18, 2026

In Vivo Monitoring of Transcriptional Activity During Metabolic Transition Using a Bioluminescent Reporter in Yeast
Published on: February 21, 2025
Overexpression of O-methyltransferase leads to improved vanillin production in baker's yeast only when complemented
Ana Rita Brochado1, Kiran R Patil
1Department of Systems Biology, Center for Microbial Biotechnology, Technical University of Denmark, Lyngby, Denmark.
Abstract:
Overproduction of a desired metabolite is often achieved via manipulation of the pathway directly leading to the product or through engineering of distant nodes within the metabolic network. Empirical examples illustrating the combined effect of these local and global strategies have been so far limited in eukaryotic systems. In this study, we compared the effects of overexpressing a key gene in de novo vanillin biosynthesis (coding for O-methyltransferase, hsOMT) in two yeast strains, with and without model-guided global network modifications. Overexpression of hsOMT resulted in increased vanillin production only in the strain with model-guided modifications, exemplifying advantage of using a global strategy prior to local pathway manipulation.
Related Concept Videos
Bioreactor Controls-III
Yeast Signaling
Microbial Fermentation

