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Updated: May 18, 2026

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

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Published on: January 18, 2017

Measuring Ca²⁺ changes in multiwell format using the Fluorometric Imaging Plate Reader (FLIPR(®)).

Ian C B Marshall¹, Davina E Owen, Shaun McNulty

¹Neurology & GI Centre of Excellence for Drug Discovery, GlaxoSmithKline Research and Development Limited, Harlow, Essex, UK.

Methods in Molecular Biology (Clifton, N.J.)

|September 26, 2012

Summary

This summary is machine-generated.

The Fluorometric Imaging Plate Reader (FLIPR) enables rapid, simultaneous measurement of cellular responses in 96- or 384-well plates. This high-throughput screening tool is crucial for drug discovery, particularly for analyzing intracellular Ca(2+) dynamics.

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Area of Science:

Pharmacology
Biotechnology
Cellular Biology

Background:

The Fluorometric Imaging Plate Reader (FLIPR) is a key technology in modern drug discovery.
Conventional plate readers lack the temporal resolution and multi-well capabilities of FLIPR.
FLIPR's ability to measure dynamic intracellular changes is essential for functional assays.

Purpose of the Study:

To describe generic methods for assessing intracellular Ca(2+) using the FLIPR system.
To highlight the advantages of FLIPR in drug discovery screening.
To explain FLIPR's utility in characterizing compound libraries.

Main Methods:

Simultaneous fluorescence emission measurement from 96- or 384-well plates.
High temporal resolution recording of dynamic intracellular processes (e.g., Ca(2+) flux, membrane potential, pH).
Application in functional assays to distinguish agonists and antagonists.

Main Results:

FLIPR facilitates rapid distinction between full agonists, partial agonists, and antagonists.
The system is valuable for interrogating large compound libraries.
Automated FLIPR systems enhance ultra-high throughput screening capabilities.

Conclusions:

FLIPR significantly contributes to drug discovery by enabling efficient screening of functional cellular responses.
Its capacity for simultaneous, high-temporal-resolution measurements makes it superior to conventional plate readers.
The described methods provide a foundation for utilizing FLIPR in Ca(2+) flux assays.