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Live Calcium Imaging of Virus-Infected Human Intestinal Organoid Monolayers Using Genetically Encoded Calcium Indicators
Published on: January 19, 2024
Compartmentalizing genetically encoded calcium sensors
David A Williams1, Mastura Monif, Kate L Richardson
1Department of Physiology, The University of Melbourne, Melbourne, VIC, Australia. davidaw@unimelb.edu.au
Methods in Molecular Biology (Clifton, N.J.)
|September 26, 2012
Summary
Researchers are using advanced genetically encoded calcium probes to understand intracellular calcium signaling. This study focuses on endoplasmic reticulum targeted probes for cell signaling in astrocytes.
Area of Science:
- Cellular biology
- Neuroscience
- Biochemistry
Background:
- Intracellular calcium ions are crucial signaling molecules modulating numerous physiological processes within single cells.
- Understanding sensitive and spatially restricted calcium changes is vital for biological research.
- Genetically encoded calcium probes offer advanced methods for detecting these changes in specific organelles.
Purpose of the Study:
- To review available fluorescent probes for intracellular calcium detection.
- To emphasize endoplasmic reticulum (ER) targeted calcium biosensors for cell signaling studies.
- To detail experimental protocols and data interpretation for these probes in astrocytes.
Main Methods:
- Utilizing genetically encoded fluorescent calcium probes.
- Targeting specific organelles like the endoplasmic reticulum, mitochondria, and nucleus.
- Employing astrocytes for cell signaling studies.
Main Results:
- Demonstrated the utility of ER-targeted calcium biosensors in astrocytes.
- Provided detailed experimental protocols for probe application.
- Outlined methods for interpreting data from calcium probes.
Conclusions:
- Genetically encoded calcium probes, particularly ER-targeted ones, are powerful tools for studying cell signaling.
- These probes enhance the understanding of calcium dynamics in organelles.
- The described methods facilitate research on astrocyte function and calcium-mediated processes.
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