Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Bioreactor Controls-II

Bioreactor Controls-II

In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...

Batch vs Continuous Culture

Batch vs Continuous Culture

Fermentation is a foundational biotechnological process used to produce pharmaceuticals, biofuels, enzymes, and food additives. Among industrial strategies, batch and continuous fermentation are the two most widely applied. Although both rely on microbial conversion of substrates into desired products, they differ markedly in operation, productivity, and suitability for specific applications.Batch fermentation occurs in a closed system in which nutrient media and inoculum are added at the...

Bioreactor Design and Operational System

Bioreactor Design and Operational System

Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...

Upstream Processing

Upstream Processing

Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

FOXD3-mediated transactivation of ALKBH5 promotes neuropathic pain via m<sup>6</sup>A-dependent stabilization of 5-HT3A mRNA in sensory neurons.

Proceedings of the National Academy of Sciences of the United States of America·2024

Same author

Arctigenin promotes mucosal healing in ulcerative colitis through facilitating focal adhesion assembly and colonic epithelial cell migration via targeting focal adhesion kinase.

International immunopharmacology·2024

Same author

Deep Reinforcement Learning for Autonomous Driving with an Auxiliary Actor Discriminator.

Sensors (Basel, Switzerland)·2024

Same author

CRISPR/Cas detection with nanodevices: moving deeper into liquid biopsy.

Chemical communications (Cambridge, England)·2024

Same author

Atomic observation and structural evolution of covalent organic framework rotamers.

Proceedings of the National Academy of Sciences of the United States of America·2024

Same author

Independent and interaction effects of prenatal exposure to high AQI and extreme Humidex on the risk of preterm birth: A large sample population study in northern China.

Reproductive toxicology (Elmsford, N.Y.)·2024

Search research articles

Related Experiment Video

Updated: May 18, 2026

Bioreactor Assembly for Continuous Culture of Complex Fecal Communities

Bioreactor Assembly for Continuous Culture of Complex Fecal Communities

Published on: April 25, 2025

Anammox start-up in sequencing batch biofilm reactors using different inoculating sludge.

Ying-Cui Yu¹, Da-Wen Gao, Yu Tao

¹College of Natural Resources and Environment, Northwest A&F University, Yangling 712100 Shaanxi, China.

Applied Microbiology and Biotechnology

|September 26, 2012

Summary

This study rapidly enriched anammox bacteria in sequencing batch biofilm reactors (SBBRs) using different inoculations. SBBRs proved effective for nitrogen removal, highlighting their potential for wastewater treatment applications.

More Related Videos

A Set of In Situ Informed Simulated Medium Formats for Culturing Environmentally Acquired Anaerobic Microorganisms

A Set of In Situ Informed Simulated Medium Formats for Culturing Environmentally Acquired Anaerobic Microorganisms

Published on: January 12, 2024

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities

Published on: December 25, 2015

Related Experiment Videos

Last Updated: May 18, 2026

Bioreactor Assembly for Continuous Culture of Complex Fecal Communities

Bioreactor Assembly for Continuous Culture of Complex Fecal Communities

Published on: April 25, 2025

A Set of In Situ Informed Simulated Medium Formats for Culturing Environmentally Acquired Anaerobic Microorganisms

A Set of In Situ Informed Simulated Medium Formats for Culturing Environmentally Acquired Anaerobic Microorganisms

Published on: January 12, 2024

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities

Published on: December 25, 2015

Area of Science:

Environmental Microbiology
Wastewater Treatment Engineering
Bioreactor Technology

Background:

Anammox (anaerobic ammonia oxidation) bacteria are crucial for efficient nitrogen removal in wastewater treatment.
Optimizing the enrichment and start-up of anammox processes is vital for practical applications.
Sequencing batch biofilm reactors (SBBRs) offer a promising platform for microbial community development.

Purpose of the Study:

To investigate the rapid enrichment of anammox bacteria in SBBRs.
To compare the performance of SBBRs with different sludge inoculations for nitrogen removal.
To analyze the impact of inoculation source on anammox activity, biofilm characteristics, and microbial community structure.

Main Methods:

Enrichment of anammox bacteria in two SBBRs with distinct inocula: activated sludge (SBBR1) and stored anaerobic sludge (SBBR2).
Evaluation of nitrogen removal performance under varying nitrogen loading rates (NLR).
Characterization of biofilm properties and microbial community dynamics using scanning electron microscopy (SEM), fluorescence in situ hybridization (FISH), and terminal restriction fragment length polymorphism (T-RFLP).

Main Results:

SBBR1 achieved a maximum NLR of 1.62 kgN/(m³/day) with >88% nitrogen removal efficiency.
SBBR2 reached a maximum NLR of 1.43 kgN/(m³/day) with 86% removal efficiency, demonstrating greater stability.
Molecular analyses revealed distinct dominant anammox bacterial genera in each reactor, influenced by the inoculation source.

Conclusions:

SBBRs are effective bioreactors for the rapid start-up and enrichment of anammox bacteria.
The choice of sludge inoculum significantly impacts the performance and microbial community composition in anammox-enriched SBBRs.
This research supports the use of SBBRs as a viable technology for enhancing nitrogen removal in wastewater treatment.