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Proteome variation among Filifactor alocis strains.

A Wilson Aruni1, Francis Roy, Lawrence Sandberg

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|September 26, 2012
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Filifactor alocis, a marker organism for periodontal disease, exhibits varied virulence. Differences in gene expression and cell wall proteins may explain its pathogenic potential, especially when coinfected with Porphyromonas gingivalis.

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Area of Science:

  • Microbiology
  • Oral Health
  • Bacterial Pathogenesis

Background:

  • Filifactor alocis is a key bacterium linked to periodontal disease.
  • This bacterium possesses virulence factors that aid its survival in periodontal pockets.
  • Strain heterogeneity exists in the virulence potential of F. alocis.

Purpose of the Study:

  • To investigate the proteome of F. alocis strains to understand pathogenesis.
  • To compare virulence factor expression between different F. alocis strains.
  • To explore the impact of coinfection with Porphyromonas gingivalis on F. alocis virulence.

Main Methods:

  • Proteomic analysis of F. alocis strains.
  • Gene expression analysis during HeLa cell invasion.
  • In silico comparative analysis of bacterial proteomes.
  • Evaluation of F. alocis gene expression during coinfection with P. gingivalis.

Main Results:

  • Proteins like proteases and neutrophil-activating protein A were identified in F. alocis.
  • The potentially more virulent F. alocis D-62D showed increased gene expression for certain proteins compared to the type strain.
  • F. alocis D-62D exhibited more cell wall anchoring proteins, with enhanced expression during coinfection with P. gingivalis.

Conclusions:

  • Differential expression of putative virulence factors contributes to the varying pathogenic potential of F. alocis strains.
  • Coinfection with P. gingivalis may enhance the expression of F. alocis virulence factors.
  • Understanding these mechanisms is crucial for addressing periodontal disease progression.