Related Experiment Video
Updated: May 18, 2026
Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation
Published on: September 6, 2017
Analysis of HLA-G polymorphisms in couples with implantation failure
Fabiola da Silva Nardi1, Renata Slowik, Pryscilla Fanini Wowk
1Laboratory of Immunogenetics and Histocompatibility, Genetics Department, Universidade Federal do Paraná, Curitiba, Brazil.
Problem:
HLA-G expression is related as an immune modulator of fetal-maternal tolerance, and its levels was correlated with pregnancy outcome. In a case-control study, we investigate the association between the genetic variability of the HLA-G gene and serum levels of soluble HLA-G in cases of embryo implantation failure.
Method Of Study:
Forty couples with at least two unsuccessful fresh embryo transfers (implantation failure; IF) and 83 fertile couples with at least two successful pregnancies was genotyped by sequencing-based typing. HLA-G alleles were defined by nucleotide sequence variations at exon 2, 3, and 4, and the quantification of soluble HLA-G (sHLA-G) was performed by ELISA.
Results:
There was a significant difference between the HLA-G allelic distributions between IF couples and the control couples. The HLA-G*01:03:01 allele was increased in the IF couples. There were no significant differences in the serum levels of sHLA-G in the IF and control groups.
Conclusion:
The results suggest that the distribution of HLA-G products may play a significant role in the modulation of maternal-fetal immune response.
