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Elution of antimicrobials from a cross-linked dextran gel: In vivo quantification
S K Hart1, J G Barrett, J A Brown
1Marion duPont Scott Equine Medical Center, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Leesburg, Virginia, USA.
Equine Veterinary Journal
|September 27, 2012
Summary
A novel biodegradable gel effectively delivers antimicrobials locally in horses, remaining above inhibitory concentrations for over 8 days. This safe, cross-linked dextran gel shows promise for treating equine infections.
Area of Science:
- Veterinary Medicine
- Pharmacology
- Biomaterials Science
Background:
- Local antimicrobial delivery is crucial for treating equine infections.
- Biodegradable gels offer a promising alternative for sustained drug release.
- Novel cross-linked dextran gel evaluated for antimicrobial elution and wound healing in horses.
Purpose of the Study:
- To determine in vivo antimicrobial elution from cross-linked dextran gel in horses.
- To evaluate the effect of implanted gel on subcutaneous wound healing in horses.
Main Methods:
- Amikacin, vancomycin, or amikacin/clindamycin-impregnated gel implanted subcutaneously in horses.
- Interstitial fluid collected via ultrafiltration probes at various time points.
- Antimicrobial concentrations analyzed using fluorescence polarization immunoassay and HPLC.
- Histological evaluation of biopsy samples to assess wound healing.
Main Results:
- Sustained antimicrobial elution detected, with concentrations remaining above minimum inhibitory levels for ≥8 days.
- Peak concentrations varied by antimicrobial: amikacin at 4h, vancomycin and clindamycin at 8h.
- No significant differences in histomorphological scores between treatment and control groups, indicating no adverse effects on wound healing.
Conclusions:
- Cross-linked dextran gel is a safe and effective local antimicrobial delivery system for horses.
- This method provides a viable alternative for managing localized infections in equine patients.
- Further research can explore broader applications in equine wound management.
