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Updated: May 18, 2026

An Optimized Rhizobox Protocol to Visualize Root Growth and Responsiveness to Localized Nutrients
Published on: October 22, 2018
Report: Comprehensive nutrients analysis of rhizomes of Polygonatum verticillatum
Haroon Khan1, Muhammad Saeed, Naveed Muhammad
1Gandhara College of Pharmacy, Gandhara University, Peshawar, Pakistan. hkdr2006@gmail.com
Abstract:
The current study was undertaken to estimate the concentration of micronutrients (Zn, Fe, Pb, Cu, Ni, Cd, Cr, Co, Sb and Mn), macronutrients (Na, Ca and K) and essential life nutrients (proteins, fats, carbohydrates and ascorbic acid) along with ash, fiber and moisture contents. Atomic absorption spectrophotometer was employed for the analysis of micronutrients while flame photometry for macronutrients. For proximate analysis (proteins, fats, carbohydrates, ash, fibers and moisture), Association of Official Analytical Chemists methods (AOAC) were used and titration method for ascorbic acid determination. It is evident from the results that the crude extract and its fractions accumulate significant concentrations of both micro and macro nutrients. The significant quantities of essential life nutrients like proteins, fats, carbohydrates and ascorbic acid along with ash, fiber and moisture contents were also found in extracts. It is concluded that the extracts of rhizomes accumulated significant quantities of life indispensible nutrients and validated the ethnobotanical uses of the plant as tonic and energizer.
