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Updated: May 18, 2026
Single Synapse Indicators of Glutamate Release and Uptake in Acute Brain Slices from Normal and Huntington Mice
Published on: March 11, 2020
Dopaminergic axons preferentially innervate dendritic spines with hyperactive glutamatergic synapses in the rat
Changlei Xu1, Junpeng Zhao, Yujun Liu
1Beijing Institute for Neuroscience, Beijing Center of Neural Regeneration and Repairing, Key Laboratory for Neurodegenerative Diseases of the Ministry of Education, Capital Medical University, Beijing 100069, PR China.
Abstract:
Dopaminergic and glutamatergic afferents simultaneously innervate median spiny neurons (MSNs) and interact to mediate basal ganglia functions. However, the association between dopaminergic and glutamatergic axons is not clear. In the present study, nigrostriatal, corticostriatal, and thalamostriatal axons were anterogradely traced with biotinylated dextran amines (BDA) in rats, and MSNs were labeled with chloromethylbenzamido-DiI for neurogeometric analysis. Results showed that nigrostriatal, but not corticostriatal or thalamostriatal, axons were biased to a target on dendritic spines of the MSNs. In addition, the MSN dendritic spines, which were innervated by tyrosine hydroxylase-immunoreactive (TH-IR) axons and vesicular glutamate transporter 1 or 2-immunoreactive (VGluT-IR) terminals, were significantly larger than dendritic spines innervated by VGluT-IR terminals alone. Under electron microscopy, glutamatergic synapses on the dendritic spines were located with TH-IR terminals and displayed longer postsynaptic density. In addition, these synapses were more perforated than those on dendritic spines lacking innervated TH-IR terminals. These results demonstrated that dopaminergic axons were biased to a target and preferred to innervate dendritic spines with hyperactive and high-efficacy glutamatergic synapses in the striatum.
