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Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors
Published on: September 10, 2013
Cellular imaging using biocompatible dendrimer-functionalized graphene oxide-based fluorescent probe anchored with
Prateek S Wate1, Shashwat S Banerjee, Archana Jalota-Badhwar
1NCE-Polymer Chemistry Group, Piramal Healthcare Ltd, Goregaon, Mumbai-400063, India.
Nanotechnology
|September 27, 2012
Summary
We developed a novel graphene nanostructure for bioimaging and drug delivery. This biocompatible system is magnetic, fluorescent, and non-toxic, showing promise for cancer cell applications.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Cancer Research
Background:
- Graphene oxide (GO) offers a versatile platform for developing multifunctional nanomaterials.
- Biocompatible and responsive nanocarriers are crucial for advanced biomedical applications like bioimaging and drug delivery.
Purpose of the Study:
- To synthesize and characterize a novel multicomponent graphene nanostructured system.
- To evaluate its potential for cellular bioimaging and delivery of bioactives.
- To assess its biocompatibility and cellular uptake kinetics.
Main Methods:
- Covalent attachment of Fe(3)O(4) nanoparticles, PAMAM-G4-NH(2) dendrimer, and Cy5 dye onto a graphene oxide surface.
- Characterization of the resulting GO-G4-Fe-Cy nanosystem for dispersion, magnetic responsiveness, and fluorescence.
- In vitro evaluation of cellular uptake by MCF-7 breast cancer cells and cytotoxicity assays on MDA-MB-231 cells.
Main Results:
- The GO-G4-Fe-Cy nanosystem demonstrated good aqueous dispersion, superparamagnetic properties, and strong NIR optical absorbance.
- Successful and stable fluorescent labeling of MCF-7 breast cancer cells was achieved.
- The nanostructured system exhibited non-toxicity to MDA-MB-231 cells, unlike free dendrimer or GO-G4 conjugates.
Conclusions:
- The developed graphene nanostructured system is biocompatible, multifunctional, and suitable for cellular bioimaging.
- Its unique properties suggest potential for targeted delivery of bioactives.
- Graphene-based nanostructures represent a promising avenue for advancing cancer diagnostics and therapeutics.
