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Updated: May 18, 2026
Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads
Published on: June 28, 2024
Preparation of high-performance water-soluble quantum dots for biorecognition through fluorescence resonance energy
Chin-Ping Huang1, Cheng-Fu Chao, Mo-Yuan Shen
1Nanotechnology Research Center, Nano-Instrumentation and Application Division, Industrial Technology Research Institute, 195, Sec. 4, Chung Hsing Rd., Chutung, Hsinchu, Taiwan 31040, ROC.
Abstract:
An improved method for the synthesis of high-performance and water-soluble quantum dots (QDs) involving the encapsulation of mercaptosuccinic acid coated QDs (MSA-QDs) with poly(diallyldimethylammonium chloride) (PDDA) followed by their direct photoactivation with fluorescent radiation near 295 K to yield PDDA-coated QDs (PDDA-QDs) has been demonstrated. The quantum yield (QY) of the PDDA-QDs was significantly improved from 0.6 (QY of MSA-QDs) to 48%. By using this synthetic strategy, highly photoluminescent PDDA-QDs of varied size were readily prepared. The surface properties of PDDA-QDs and MSA-QDs were extensively characterized. The highly luminescent and positively charged PDDA-QDs serve as a useful and convenient tool for protein adsorption. With a Δ(5)-3-ketosteroid isomerase adsorbed PDDA-QD complex, the biorecognition of steroids was demonstrated through the application of fluorescent resonance energy transfer.
