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Updated: May 18, 2026

Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus
Published on: October 7, 2011
The role of equine herpesvirus type 4 glycoprotein k in virus replication
Walid Azab1, Abuelyazeed El-Sheikh
1Institute of Virology, Department of Veterinary Medicine, Free University of Berlin, Philippstrasse 13, Haus 18, 10115 Berlin, Germany. wfazab@zedat.fu-berlin.de
Abstract:
Equine herpesvirus 4 (EHV-4) is an important equine pathogen that causes respiratory tract disease among horses worldwide. Glycoprotein K (gK) homologues have been identified in several alphaherpesviruses as a major player in virus entry, replication, and spread. In the present study, EHV-4 gK-deletion mutant has been generated by using bacterial artificial chromosome technology and Red mutagenesis to investigate the role of gK in EHV-4 replication. Our findings reported here show that gK is essential for virus replication in vitro and that the gK-negative strain was not able to be reconstituted in equine cells. It is noteworthy that these findings agree with the previously published study describing gK deletion in other alphaherpesviruses.
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