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Related Experiment Video

Updated: May 18, 2026

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone

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Published on: February 23, 2017

Tunable ionic mobility filter for depletion zone isotachophoresis.

Jos Quist¹, Paul Vulto, Heiko van der Linden

¹Leiden/Amsterdam Centre for Drug Research (LACDR), Division of Analytical Biosciences, Einsteinweg 55, 2333CC, Leiden, The Netherlands.

Analytical Chemistry

|September 28, 2012

Summary

We developed a novel tunable filter using depletion zone isotachophoresis (dzITP) for selective compound release. This micro/nanofluidic device enhances analyte detection and enrichment from complex samples like urine.

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Published on: July 24, 2017

Area of Science:

Micro/nanofluidics
Analytical Chemistry
Biochemical Assays

Background:

Isotachophoresis (ITP) is a powerful separation technique.
Existing ITP methods lack tunable filtering capabilities for complex sample matrices.

Purpose of the Study:

To introduce a novel tunable low-pass ionic mobility filter based on depletion zone isotachophoresis (dzITP).
To demonstrate quantitative control over compound release and analyte enrichment using dzITP.
To showcase the application of dzITP filtering in enhancing detection and extraction from biological samples.

Main Methods:

Development of a micro/nanofluidic filter utilizing dzITP principles.
Implementation of current and voltage actuation for controlled compound release.
Demonstration of continuous and pulsed modes of operation for selective analyte manipulation.
Application of the filter for enhancing the detection of fluorescent compounds and enriching analytes from diluted urine.

Main Results:

Realization of a tunable low-pass ionic mobility filter.
Quantitative control of fluorescent compound release demonstrated via electrical actuation.
4-fold enhancement in detecting 6-carboxyfluorescein over fluorescein, despite a 250x lower concentration.
Successful extraction and enrichment of high-mobility analytes from diluted raw urine samples.

Conclusions:

Depletion zone isotachophoresis (dzITP) enables a novel, tunable ionic filtering mechanism.
The dzITP filter offers precise control over analyte separation and release in micro/nanofluidic systems.
This technology provides a versatile toolkit for enhancing detection and performing pre-concentration of analytes in biochemical assays and complex sample analysis.