Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Hypersensitivity Reactions: Delayed Hypersensitivity Reactions01:29

Hypersensitivity Reactions: Delayed Hypersensitivity Reactions

Delayed-Type Hypersensitivity (DTH), or Type IV hypersensitivity, is a cell-mediated immune response. It occurs when T cells, rather than antibodies, mediate a reaction to specific antigens. It is characterized by a delayed onset (1-2 days) and involves the recruitment of macrophages to the inflammation site.The initiation of a DTH response begins with the sensitization of T cells. During this phase, which lasts at least 1-2 weeks, antigen-specific T cells are activated, clonally expanded, and...
Drug Toxicity: Allergic Reactions01:30

Drug Toxicity: Allergic Reactions

Drug-related allergies are immune-mediated responses triggered by the administration of pharmacological agents. These hypersensitivity reactions are classified based on the immune mechanisms involved. The four primary types—Type I, II, III, and IV—are mediated by different immunological pathways and exhibit distinct clinical manifestations.Type I Hypersensitivity/ IgE-Mediated Reactions: Immunoglobulin E (IgE) immediately mediates Type I hypersensitivity reactions. Upon initial exposure to a...
Hypersensitivities01:30

Hypersensitivities

Hypersensitivity, also known as a hypersensitivity reaction or allergic reaction, is a condition where the body's immune system reacts abnormally to a foreign substance. Such substances, that cause hypersensitivity are referred to as an allergen, could be something typically harmless to most people, like pollen or certain foods.
Types of Hypersensitivities
Hypersensitivity reactions are categorized into four types: Type 1, Type 2, Type 3, and Type 4. Each type has a distinct mechanism...
Hypersensitivity Reactions: Immune-Complex Reactions01:19

Hypersensitivity Reactions: Immune-Complex Reactions

Type III hypersensitivity reactions occur when antigen–antibody complexes form and activate the complement system. Normally, these complexes help the clearance of antigens by phagocytes and red blood cells. However, when large numbers of immune complexes are present, they can deposit in tissues—particularly in the walls of blood vessels—leading to inflammation and tissue injury. These deposits trigger complement activation and neutrophil recruitment, resulting in serum sickness, a systemic...
Allergic Drug Reactions01:27

Allergic Drug Reactions

Allergic reactions related to drugs are hypersensitivity responses driven by the immune system and bear no connection to the drug's therapeutic action. While drugs in isolation do not trigger an immune response, they can interact with endogenous proteins to form antigens. These antigens stimulate lymphocytes to produce antibodies. IgE-type antibodies attach themselves to mast cells. Upon subsequent exposure to the same stimulus, the antigen-antibody interaction is initiated, unleashing numerous...
Pulmonary Tuberculosis IV01:26

Pulmonary Tuberculosis IV

Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Assessing intestinal fibrosis using an endoscopic photoacoustic-ultrasound balloon catheter in rabbits and a human subject.

Journal of Crohn's & colitis·2025
Same author

Regulation of Parietal Cell Homeostasis by Bone Morphogenetic Protein Signaling.

Gastro hep advances·2024
Same author

Diet-driven differential response of Akkermansia muciniphila modulates pathogen susceptibility.

Molecular systems biology·2024
Same author

Opposing diet, microbiome, and metabolite mechanisms regulate inflammatory bowel disease in a genetically susceptible host.

Cell host & microbe·2024
Same author

Diet-driven differential response of <i>Akkermansia muciniphila</i> modulates pathogen susceptibility.

bioRxiv : the preprint server for biology·2024
Same author

Corrigendum to "A steamed broccoli sprout diet preparation that reduces colitis via the gut microbiota" [J Nutr Biochem 2023;112:109215].

The Journal of nutritional biochemistry·2023

Related Experiment Video

Updated: May 18, 2026

Induction and Monitoring of Active Delayed Type Hypersensitivity (DTH) in Rats
13:26

Induction and Monitoring of Active Delayed Type Hypersensitivity (DTH) in Rats

Published on: July 19, 2007

Delayed-type hypersensitivity determination.

Brian M Gray1, Kathryn A Eaton

  • 1Unit for Laboratory Animal Medicine, Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, USA.

Methods in Molecular Biology (Clifton, N.J.)
|September 28, 2012
PubMed
Summary
This summary is machine-generated.

This study details a method for assessing cell-mediated immunity (CMI) in mice using delayed-type hypersensitivity skin responses. The technique involves measuring footpad swelling 24 hours after injecting Helicobacter organisms.

More Related Videos

Trans-vivo Delayed Type Hypersensitivity Assay for Antigen Specific Regulation
11:49

Trans-vivo Delayed Type Hypersensitivity Assay for Antigen Specific Regulation

Published on: May 2, 2013

Induction and Monitoring of Adoptive Delayed-Type Hypersensitivity in Rats
22:06

Induction and Monitoring of Adoptive Delayed-Type Hypersensitivity in Rats

Published on: October 1, 2007

Related Experiment Videos

Last Updated: May 18, 2026

Induction and Monitoring of Active Delayed Type Hypersensitivity (DTH) in Rats
13:26

Induction and Monitoring of Active Delayed Type Hypersensitivity (DTH) in Rats

Published on: July 19, 2007

Trans-vivo Delayed Type Hypersensitivity Assay for Antigen Specific Regulation
11:49

Trans-vivo Delayed Type Hypersensitivity Assay for Antigen Specific Regulation

Published on: May 2, 2013

Induction and Monitoring of Adoptive Delayed-Type Hypersensitivity in Rats
22:06

Induction and Monitoring of Adoptive Delayed-Type Hypersensitivity in Rats

Published on: October 1, 2007

Area of Science:

  • Immunology
  • Microbiology

Background:

  • Cell-mediated immunity (CMI) is crucial for host defense against intracellular pathogens like Helicobacter.
  • Assessing CMI in vivo is essential for understanding immune responses to infections.

Purpose of the Study:

  • To describe a standardized method for evaluating delayed-type hypersensitivity (DTH) in mice.
  • To provide a protocol for measuring CMI against Helicobacter infection.

Main Methods:

  • The study utilizes the mouse footpad as the site for delayed-type hypersensitivity assessment.
  • Mice are inoculated with cultured Helicobacter organisms into the hind footpad.
  • Induration (swelling) at the inoculation site is measured 24 hours post-inoculation.

Main Results:

  • The described method allows for quantitative measurement of DTH responses.
  • This protocol enables in vivo assessment of CMI to Helicobacter.

Conclusions:

  • The footpad DTH assay is a reliable method for assessing CMI in mice.
  • This technique is valuable for studying immune responses in Helicobacter-infected mouse models.