Development of pH-based ElecFET biosensors for lactate ion detection

Area of Science:

• Electrochemistry
• Biosensors
• Microtechnology

Background:

• Electrochemical Field Effect Transistors (ElecFETs) integrate pH-sensitive field-effect transistors with metallic microelectrodes.
• This design enables combined potentiometric and amperometric detection at the microscale for enhanced sensing capabilities.

Purpose of the Study:

• To design, fabricate, and experimentally validate ElecFET devices for molecular detection in aqueous solutions.
• To explore the potential of ElecFETs for sensitive and selective detection of key analytes.

Main Methods:

• Utilized silicon and polymer micro-technologies for ElecFET fabrication.
• Integrated pH-sensitive chemical field-effect transistors (pH-ChemFETs) with metallic microelectrodes.
• Performed experimental validation for the detection of hydrogen peroxide, glucose, and lactate.

Main Results:

• Demonstrated hydrogen peroxide detection with a sensitivity of 5 mV/mM in the 10-100 mM range.
• Achieved glucose detection sensitivity between 2-6 mV/mM within the 1-30 mM range.
• Reported lactate detection sensitivity between 8-20 mV/mM in the 1-6 mM range.

Conclusions:

• ElecFETs are effective electrochemical microsensors for detecting various molecules in solution.
• The developed devices show promising sensitivity for analytes including hydrogen peroxide, glucose, and lactate.
• ElecFET technology offers a versatile platform for microscale potentiometric and amperometric sensing applications.