Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structural insights into allosteric regulation of GdpP: A conformationally dynamic phosphodiesterase.

Structure (London, England : 1993)·2026
Same author

A SuperSelective primer-based real-time PCR Platform for hypersensitive detection of azole heteroresistance in Cryptococcus neoformans.

bioRxiv : the preprint server for biology·2026
Same author

Maternal Inflammation Alters Nuclear and Mitochondrial DNA Methylation Patterns in Neonatal Brain Monocytes.

Cells·2026
Same author

Circuitous Ways of EWS::FLI1 Using Circular RNA ZNF609 to Evade Translational Repression by miR-145 in Ewing's Sarcoma.

Biomedicines·2026
Same author

EVRCEPT: EV RNA Cargo Enrichment Prediction Tool to predict enrichment of RNA into Extracellular Vesicles.

bioRxiv : the preprint server for biology·2025
Same author

Single-cell and spatial transcriptomics map senescent vascular cells in arterial remodeling during atherosclerosis in mice.

Nature aging·2025

Related Experiment Video

Updated: May 18, 2026

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
16:16

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

Highly bright avidin-based affinity probes carrying multiple lanthanide chelates.

Laura Wirpsza1, Shyamala Pillai, Mona Batish

  • 1Department of Chemistry and Environmental Sciences, New Jersey Institute of Technology, 151 Tiernan Hall, University Heights, Newark, NJ 07102, USA.

Journal of Photochemistry and Photobiology. B, Biology
|September 29, 2012
PubMed
Summary

Researchers developed highly bright lanthanide (Ln) chelate-avidin constructs for sensitive detection. Introducing an aromatic spacer prevented quenching in terbium (Tb3+) labels, achieving detection limits down to 10(-15) M.

More Related Videos

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

Related Experiment Videos

Last Updated: May 18, 2026

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
16:16

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

Area of Science:

  • Bioconjugation Chemistry
  • Luminescent Probes
  • Analytical Chemistry

Background:

  • Lanthanide ion luminescence offers long lifetimes for sensitive time-gated detection.
  • Increasing sensitivity involves conjugating multiple luminescent labels to carrier molecules.

Purpose of the Study:

  • To create highly luminescent bioconjugates by attaching multiple lanthanide chelates to avidin.
  • To investigate the effects of high label density on luminescence and suppress quenching in terbium (Tb3+) probes.

Main Methods:

  • Attachment of up to 30 lanthanide chelates (Eu3+ and Tb3+) to avidin.
  • Introduction of an aromatic spacer to mitigate luminescence quenching.
  • Detection limit determination and application in labeling bacterial and mammalian cells.

Main Results:

  • Avidin conjugates with up to 30 Eu3+ chelates showed enhanced brightness due to a synergistic effect.
  • Tb3+ chelates exhibited luminescence quenching at high densities, which was resolved by using an aromatic spacer.
  • The developed probes achieved ultra-low detection limits in the 10(-14)-10(-15) M range.

Conclusions:

  • Highly bright and sensitive lanthanide-based bioprobes can be constructed by optimizing label density and attachment strategies.
  • Aromatic spacers are crucial for preventing luminescence quenching in densely labeled Tb3+ constructs.
  • These novel probes demonstrate significant potential for highly sensitive bioimaging and detection applications, including live cell labeling.