Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Assembly of Cytoskeletal Filaments01:18

Assembly of Cytoskeletal Filaments

Cytoskeletal filaments are polymeric forms of smaller protein subunits. However, individual cytoskeletal filaments may easily disassemble or associate with other similar filaments to form rigid structures. Microfilaments, made of actin monomers, rely on actin-binding proteins to form bundles and create networks of individual actin filaments. Microtubules rely on microtubule-associated proteins (MAPs) to form sturdy cylindrical structures. However, the proteins involved in forming complex...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Targeting intracellular cholesterol imbalance rescues sarcomere-ER contact site signaling and ER remodeling in dilated cardiomyopathy.

Signal transduction and targeted therapy·2026
Same author

ATP synthase activity boosts membrane proton acceptance and lateral diffusion.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Minimal actin cortices: The αD-helix of ezrin's C-ERMAD governs F-actin binding.

The Journal of biological chemistry·2025
Same author

Phase-separated plasma membranes show elevated viscosity and reduced line tension of liquid-ordered domains.

Journal of colloid and interface science·2025
Same author

Tissue tension of planar, free standing cell monolayers measured by central deformation.

PNAS nexus·2025
Same author

Glycation of alpha-synuclein enhances aggregation and neuroinflammatory responses.

NPJ Parkinson's disease·2025

Related Experiment Video

Updated: May 18, 2026

Synthesis of Monodisperse Cylindrical Nanoparticles via Crystallization-driven Self-assembly of Biodegradable Block Copolymers
11:42

Synthesis of Monodisperse Cylindrical Nanoparticles via Crystallization-driven Self-assembly of Biodegradable Block Copolymers

Published on: June 20, 2019

Macromolecular shape and interactions in layer-by-layer assemblies within cylindrical nanopores.

Thomas D Lazzara1, K H Aaron Lau, Wolfgang Knoll

  • 1Institute of Organic and Biomolecular Chemistry, Tammannstr. 2, 37077 Göttingen, Germany.

Beilstein Journal of Nanotechnology
|September 29, 2012
PubMed
Summary

Layer-by-layer (LbL) deposition of polyelectrolytes and proteins in nanoporous anodic aluminum oxide (AAO) membranes showed pore size limits film growth. Proteins formed looser layers than polyelectrolytes, with growth inhibited at pore diameters similar to native AAO.

Keywords:
avidin-biotindendrimersnanoporous substratesoptical lightmode waveguide spectroscopypolyelectrolytes

More Related Videos

Controlling the Size, Shape and Stability of Supramolecular Polymers in Water
16:24

Controlling the Size, Shape and Stability of Supramolecular Polymers in Water

Published on: August 2, 2012

Functionalization of Single-walled Carbon Nanotubes with Thermo-reversible Block Copolymers and Characterization by Small-angle Neutron Scattering
09:12

Functionalization of Single-walled Carbon Nanotubes with Thermo-reversible Block Copolymers and Characterization by Small-angle Neutron Scattering

Published on: June 1, 2016

Related Experiment Videos

Last Updated: May 18, 2026

Synthesis of Monodisperse Cylindrical Nanoparticles via Crystallization-driven Self-assembly of Biodegradable Block Copolymers
11:42

Synthesis of Monodisperse Cylindrical Nanoparticles via Crystallization-driven Self-assembly of Biodegradable Block Copolymers

Published on: June 20, 2019

Controlling the Size, Shape and Stability of Supramolecular Polymers in Water
16:24

Controlling the Size, Shape and Stability of Supramolecular Polymers in Water

Published on: August 2, 2012

Functionalization of Single-walled Carbon Nanotubes with Thermo-reversible Block Copolymers and Characterization by Small-angle Neutron Scattering
09:12

Functionalization of Single-walled Carbon Nanotubes with Thermo-reversible Block Copolymers and Characterization by Small-angle Neutron Scattering

Published on: June 1, 2016

Area of Science:

  • Materials Science
  • Nanotechnology
  • Biomaterials Engineering

Background:

  • Layer-by-layer (LbL) assembly is a versatile technique for fabricating multilayered thin films.
  • Anodic aluminum oxide (AAO) membranes offer a tunable nanoporous template for controlled material deposition.
  • Understanding macromolecular behavior within confined geometries is crucial for advanced material design.

Purpose of the Study:

  • To investigate the in situ growth dynamics of LbL films composed of polyelectrolytes and proteins within AAO nanopores.
  • To compare the LbL deposition behavior of linear polyelectrolytes (linear-PEs) and globular proteins within varying AAO pore sizes.
  • To determine the physical limitations and structural characteristics of LbL multilayer formation in cylindrical nanopores.

Main Methods:

  • Optical waveguide spectroscopy (OWS) for real-time monitoring of LbL deposition within AAO.
  • Fabrication of AAO membranes with controlled pore diameters (25-80 nm).
  • Scanning electron microscopy (SEM) for post-deposition structural analysis.

Main Results:

  • LbL film growth was inhibited at a maximum number of steps (n(max)) that varied between linear-PEs and proteins.
  • Proteins exhibited lower n(max) values compared to linear-PEs, indicating different packing densities.
  • Deposition was inhibited in native AAO pores (25-30 nm) and larger pores (d(0) > 25-30 nm) when the effective pore diameter approached 25-35 nm.
  • OWS and SEM confirmed that LbL growth inhibition is linked to pore geometry and macromolecular packing.

Conclusions:

  • The cylindrical pore geometry of AAO imposes a physical constraint on LbL multilayer growth.
  • Macromolecular packing density significantly influences the maximum achievable multilayer thickness within nanopores.
  • Electrostatic LbL resulted in compact layers for polyelectrolytes, while proteins formed loosely packed multilayers, impacting growth limitations.