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Updated: May 18, 2026

Measuring Local Anaphylaxis in Mice
Published on: October 14, 2014
[Relationship between specific immunocyte and pseudoanaphylactoid reactions induced by Shuanghuanglian injection]
Yan Yi1, Aihua Liang, Chunying Li
1Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. yiyan800109@yahoo.com.cn
Objective:
To investigate the relationship between specific immunocyte and pseudoanaphylactoid reactions (PR) induced by Shuanghuanglian injection (SHLI).
Method:
ICR mice, SCID mice and BALB/C athymic mouse were divided into different test groups, the mice were intravenously injected with solutions of different concentration of SHLI, positive control Compound 48/80 and normal sodium. All test substances were mixed with 0. 4% Evans blue. The reaction and vascular permeability of the ears were observed and measured 30 min after SHLI injected.
Result:
SHLI of 300, 600 mg x kg(-1) caused obvious vascular hyperpermeability in ICR mice, but the same dose of SHLI didn't cause vascular hyperpermeability in SCID mice and BALB/C athymic mouse.
Conclusion:
SHLI in equivalent and 2 times the clinical dose can cause PR in ICR mice, but the same dose of SHLI can't cause PR in SCID mice and BALB/C athymic mouse, so specific immunocyte maybe take part in the SHLI-induced PR.
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