Related Experiment Video
Updated: May 18, 2026

Formation of Dispersible Taohong Siwu Tablets
Published on: February 3, 2023
[Study on absorption ingredients of yuanhuzhitong pill by everted intestinal sac method]
Xiaomeng Chen1, Yingchun Zhang, Shuo Lin
1College of life science and engineering, Southwest Jiaotong University, Chengdu 610031, China. juying169@yahoo.com.cn
Objective:
To establish an everted intestinal sac method for determining absorption ingredients of yuanhuzhitong pill and study absorption characteristics of major chemical constituents of yuanhuzhitong pill.
Method:
The everted intestinal sac model was adopted. Intestinal sac fluid samples at different time points after administration of three concentrations of Yuanhuzhitong pill were collected and determined by HPLC. The accumulative absorbed doses of active constituents were calculated, while the proportion between samples of yuanhuzhitong pill and absorption ingredients was compared.
Result:
Eight ingredients of Yuanhuzhitong pill can be detected in intestinal sac, they are protopine, palmatine, coptisine, imperatorin, berberine, byakangelicin, alpha-allocryptopine and tetrahydropalmatine. The absorption rate constants (Ka) of eight constituents increased in jejunum and ileum with the increase in concentration of Yuanhuzhitong pill extracts (P < 0. 05), indicating a passive absorption.
Conclusion:
As ingredients are selectively absorbed in intestinal sac, the everted intestinal sac method is selected to assess the intestinal absorption characteristics of ingredients of Yuanhuzhitong prescription.
Related Concept Videos
Methods for Studying Drug Absorption: In vitro
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
Methods for Studying Drug Absorption: In situ
The Doluisio method involves perfusing a prepared segment of a rat's small intestine with a solution of radiolabeled drug and a non-absorbable marker. This helps to differentiate between absorbed and non-absorbed drug concentrations. The intestinal segment is connected at both ends using tubing and syringes,...
Glucose Absorption Into the Small Intestine
Factors Influencing Drug Absorption: Anatomical Parameters
In Vitro Drug Dissolution: Alternative Methods
Drug Absorption: Factors Affecting GI Absorption
In...
