Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Drug Delivery: Miscellaneous Routes01:22

Drug Delivery: Miscellaneous Routes

Drug delivery methods like oral inhalation, nasal sprays, transdermal patches, eye drops, intravitreal injection,  and rectal administration provide localized effects with reduced toxicity.
Oral inhalation and nasal sprays swiftly transfer drugs across the respiratory epithelium's mucosal layer. Inhaled glucocorticoids and bronchodilators directly target lung conditions such as asthma, while fluticasone nasal spray mitigates allergic rhinitis.
Transdermal patches transport drugs through the...
Solvents01:12

Solvents

A solvent is a substance, most often a liquid, that can dissolve other substances. Here, the substance being dissolved is called a solute. When a solvent and a solute combine, they form a solution - a homogenous mixture of both the solvent and the solute. Water is a universal biological solvent. Its polar structure allows it to dissolve many other polar compounds. The ability of water to dissolve is governed by a balance between water molecules binding to each other and binding to the solute.
A...
Antiasthma Drugs: Inhaled Corticosteroids and Glucocorticoids01:25

Antiasthma Drugs: Inhaled Corticosteroids and Glucocorticoids

Inhaled corticosteroids (ICS) are anti-inflammatory drugs used primarily in treating persistent asthma and providing long-term maintenance. They target the bronchial mucosa, the lining of the airways, to control inflammation, a critical factor in asthma progression and exacerbation.
ICS work through a multifaceted mechanism of action. They suppress the inflammatory response caused by the proliferation of TH cells. They also reduce the transcription of the IL-2 gene, which is involved in the...
Drugs for Treatment of Crohn's Disease in IBD Using Glucocorticoids01:21

Drugs for Treatment of Crohn's Disease in IBD Using Glucocorticoids

Glucocorticoids, a class of anti-inflammatory drugs, are pivotal in treating moderate to severe Crohn's disease by inducing remission. They exhibit their anti-inflammatory action by inhibiting the production of inflammatory cytokines such as tumor necrosis factor (TNF)-α, interleukin (IL)-1, and chemokines like IL-8. In addition, they reduce the expression of inflammatory cell adhesion molecules and inhibit gene transcription of nitric oxide synthase, phospholipase A2, cyclooxygenase-2 (COX-2),...
Ophthalmic Drug Delivery Systems01:23

Ophthalmic Drug Delivery Systems

Ophthalmic drug delivery faces major limitations due to poor absorption across the corneal membrane. This process is primarily driven by diffusion and is influenced by two main factors: the physicochemical properties of the drug and tear drainage. Most ophthalmic drugs, such as pilocarpine, epinephrine, atropine, and local anesthetics, are weak bases. They are typically formulated at an acidic pH to enhance chemical stability. However, this leads to high ionization, reducing their ability to...
Local Anesthetics: Common Agents and Their Applications01:23

Local Anesthetics: Common Agents and Their Applications

Local anesthetics (LAs) are commonly used for various applications in medical and dental procedures. Some of the common agents used are cocaine, lidocaine, and bupivacaine.
Cocaine is an ester of benzoic acid and methylecgogine. It is used to anesthetize and vasoconstrict locally. Currently, it is used primarily for topical applications. It is beneficial for surgeries on the upper respiratory tract, providing anesthesia and shrinking the mucosa. Cocaine in the form of cocaine hydrochloride is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Leukaemic infiltration and cytomegalovirus retinitis in a patient with acute T-cell lymphoblastic leukaemia in complete remission.

Archivos de la Sociedad Espanola de Oftalmologia·2016
Same author

[Diabetic retinopathy screening and teleophthalmology].

Archivos de la Sociedad Espanola de Oftalmologia·2012
Same author

[The hazy frontier between fraud and scientific truth].

Archivos de la Sociedad Espanola de Oftalmologia·2006
Same author

[Juvenile Diabetic cataract. A rare finding which lead us to the diagnosis of this illness].

Archivos de la Sociedad Espanola de Oftalmologia·2003
Same journal

Extra-ocular muscle haematoma masquerading uveal prolapse post blunt trauma to the eye: A case report.

Archivos de la Sociedad Espanola de Oftalmologia·2026
Same journal

Multifocal intraocular lenses in refractive lens exchange: Guidelines to optimize their use and indications.

Archivos de la Sociedad Espanola de Oftalmologia·2026
Same journal

The Effect of Pre- and Perioperative Anxiety on Cardiovascular Parameters and Intraocular Pressure of Patients Awaiting Phacoemulsification Surgery.

Archivos de la Sociedad Espanola de Oftalmologia·2026
Same journal

Management of central corneal opacities using manual and femtosecond laser-assisted rotational anterior lamellar autokeratoplasty: A descriptive case series.

Archivos de la Sociedad Espanola de Oftalmologia·2026
Same journal

Evaluation of the anterior hyaloid using intraoperative optical coherence tomography: a key strategy in complex cataract surgery.

Archivos de la Sociedad Espanola de Oftalmologia·2026
Same journal

Unilateral ocular hypertension secondary to frontal mucocele with orbital extension.

Archivos de la Sociedad Espanola de Oftalmologia·2026
See all related articles

Related Experiment Video

Updated: May 18, 2026

Subretinal Implantation of RPE on a Carrier in Minipigs: Guidelines for Preoperative Preparations, Surgical Techniques, and Postoperative Care
11:06

Subretinal Implantation of RPE on a Carrier in Minipigs: Guidelines for Preoperative Preparations, Surgical Techniques, and Postoperative Care

Published on: November 11, 2022

[Triamcinolone acetonide without solvents].

M Martínez Rubio1, M Moya Moya, J Selva Otaola-Urruchi

  • 1Servicio de Oftalmología, Hospital General Universitario Alicante, Alicante, España. martinez_magrub@gva.es

Archivos De La Sociedad Espanola De Oftalmologia
|October 2, 2012
PubMed
Summary
This summary is machine-generated.

This study introduces a new sterile method for preparing pure triamcinolone acetonide without preservatives, crucial for avoiding benzyl alcohol toxicity in ophthalmic preparations.

More Related Videos

The Goeckerman Regimen for the Treatment of Moderate to Severe Psoriasis
11:39

The Goeckerman Regimen for the Treatment of Moderate to Severe Psoriasis

Published on: July 11, 2013

Primed Mycobacterial Uveitis (PMU) as a Model for Post-Infectious Uveitis
10:33

Primed Mycobacterial Uveitis (PMU) as a Model for Post-Infectious Uveitis

Published on: December 17, 2021

Related Experiment Videos

Last Updated: May 18, 2026

Subretinal Implantation of RPE on a Carrier in Minipigs: Guidelines for Preoperative Preparations, Surgical Techniques, and Postoperative Care
11:06

Subretinal Implantation of RPE on a Carrier in Minipigs: Guidelines for Preoperative Preparations, Surgical Techniques, and Postoperative Care

Published on: November 11, 2022

The Goeckerman Regimen for the Treatment of Moderate to Severe Psoriasis
11:39

The Goeckerman Regimen for the Treatment of Moderate to Severe Psoriasis

Published on: July 11, 2013

Primed Mycobacterial Uveitis (PMU) as a Model for Post-Infectious Uveitis
10:33

Primed Mycobacterial Uveitis (PMU) as a Model for Post-Infectious Uveitis

Published on: December 17, 2021

Area of Science:

  • Ophthalmology
  • Pharmaceutical Sciences
  • Sterile Manufacturing

Context:

  • Commercially available triamcinolone acetonide often contains benzyl alcohol, a solvent with potential toxic side-effects.
  • Purification of triamcinolone acetonide is essential for safe intravitreal administration.
  • Developing preservative-free formulations is a key goal in pharmaceutical development.

Purpose:

  • To present a novel, sterile technique for preparing pure triamcinolone acetonide.
  • To eliminate the need for organic solvents like benzyl alcohol in the preparation process.
  • To establish a reliable method for producing preservative-free triamcinolone acetonide suspensions.

Summary:

  • A sterile suspension of triamcinolone acetonide was prepared using only sterile distilled water.
  • The suspension was processed under aseptic conditions and terminally sterilized via autoclaving.
  • High-pressure liquid chromatography confirmed the final concentration for intravitreal injection was 3.77mg/0.1ml.

Impact:

  • This method yields pure triamcinolone acetonide without harmful preservatives.
  • The resulting formulation is chemically, physically, and microbiologically stable for six months when refrigerated.
  • Offers a safer alternative for ophthalmic treatments requiring triamcinolone acetonide.