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Related Experiment Videos

Alpha-tocopherol consumption during low-density-lipoprotein oxidation.

W Jessup1, S M Rankin, C V De Whalley

  • 1Department of Biology and Biochemistry, Middlesex, U.K.

The Biochemical Journal
|January 15, 1990
PubMed
Summary

Alpha-tocopherol (vitamin E) in low-density lipoprotein (LDL) prevents oxidation. Supplementing LDL with alpha-tocopherol or adding flavonoids like morin inhibits LDL oxidation, but individual LDL antioxidant content varies.

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Area of Science:

  • Biochemistry
  • Cardiovascular Research
  • Nutritional Science

Background:

  • Low-density lipoprotein (LDL) oxidation is a key factor in atherosclerosis.
  • Alpha-tocopherol, an antioxidant, is present in LDL and may protect against its oxidation.
  • The role of endogenous antioxidants in LDL's resistance to oxidation requires further investigation.

Purpose of the Study:

  • To investigate the kinetics of alpha-tocopherol depletion in LDL during oxidation.
  • To determine the effect of alpha-tocopherol supplementation and flavonoid addition on LDL oxidation.
  • To assess the relationship between endogenous alpha-tocopherol levels and LDL oxidative resistance.

Main Methods:

  • Measuring alpha-tocopherol depletion in human LDL during cell-mediated and cell-free oxidation.

Related Experiment Videos

  • Supplementing LDL with alpha-tocopherol in vivo.
  • Assessing the impact of the flavonoid morin on LDL oxidation.
  • Analyzing alpha-tocopherol content in LDL from various individual donors.

    • Main Results:

    • Oxidative modification of LDL was not detected until all endogenous alpha-tocopherol was consumed.
    • In vivo alpha-tocopherol supplementation extended the lag phase before LDL oxidation.
    • The flavonoid morin inhibited both alpha-tocopherol consumption and LDL oxidation.
    • LDL alpha-tocopherol levels did not predict individual resistance to oxidation.

    Conclusions:

    • Alpha-tocopherol acts as a primary antioxidant in LDL, delaying oxidative modification.
    • Flavonoids can prevent LDL oxidation by preserving alpha-tocopherol.
    • Human LDL contains other significant endogenous antioxidants besides alpha-tocopherol.