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Categorization of near-term mouse fetuses according to their viability as a tool for developmental toxicity screening
1Institute of Anatomy, Faculty of Medicine, University of Oporto, Portugal.
In 1,015 CD-1 near-term mouse fetuses, removed by cesarean section after cervical dislocation of dams, skin color, motility, crying, and breathing were studied. Three categories of fetuses were considered: living and surviving (92.5%); living but non-surviving (6.9%), and stillborn (0.6%). Particular attention was paid to the following: 24 fetuses, still apneic after routine manipulation, started breathing by specific maneuvers; 5 of these 24 fetuses were apparently dead when apnea recovery was successfully tried, and the living but non-surviving category must be considered in developmental toxicity screening as prenatal injuries may be manifested by an increase in the incidence of the fetuses belonging to this category.
In 1,015 CD-1 near-term mouse fetuses, removed by cesarean section after cervical dislocation of dams, skin color, motility, crying, and breathing were studied. Three categories of fetuses were considered: living and surviving (92.5%); living but non-surviving (6.9%), and stillborn (0.6%). Particular attention was paid to the following: 24 fetuses, still apneic after routine manipulation, started breathing by specific maneuvers; 5 of these 24 fetuses were apparently dead when apnea recovery was successfully tried, and the living but non-surviving category must be considered in developmental toxicity screening as prenatal injuries may be manifested by an increase in the incidence of the fetuses belonging to this category.