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Updated: May 18, 2026
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Published on: December 3, 2013
Isolation and Typing of Canine Parvovirus in CRFK Cell Line in Puducherry, South India
S Parthiban1, Hirak Kumar Mukhopadhyay, D Panneer
1Department of Veterinary Microbiology, Rajiv Gandhi College of Veterinary and Animal Sciences, Puducherry, 605009 India ; 10/442, 1st Cross, Thulukathamman Nagar, Murungapakkam Post, Pondicherry, 605004 India.
Abstract:
A total of 128 faecal samples/rectal swabs were collected from dogs showing signs of diarrhea/enteritis in and around Puducherry, South India. Eighteen clinical samples, showing high HA titre of 1:512 and above and positivity by polymerase chain reaction (PCR) with CPV-2ab primers, were subjected to virus isolation in CRFK cell line. Of the 18 samples processed, 3 samples (16.6%) were positive for CPV and were confirmed by haemagglutination, dot-ELISA, and IFAT. The three cell culture isolates were characterized as CPV-2b types by multiplex PCR as well as by monoclonal antibody typing.
