Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Production of Biopesticides01:18

Production of Biopesticides

Biopesticides offer a sustainable alternative to chemical pesticides, utilizing microbial agents to control agricultural pests. Bacillus thuringiensis (Bt) is a widely employed bacterium known for its potent insecticidal activity. Bt biopesticides are favored for their specificity to insect pests, minimal environmental impact, and natural degradability.Mechanism of Bt Toxin Action Bt produces insecticidal crystal (Cry) proteins during its sporulation phase. These proteins form parasporal...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Promoting contraceptive use among unmarried female migrants in one factory in Shanghai: a pilot workplace intervention.

BMC health services research·2007
Same author

Apoptosis related protein 3, an ATRA-upregulated membrane protein arrests the cell cycle at G1/S phase by decreasing the expression of cyclin D1.

Biochemical and biophysical research communications·2007
Same author

Efficient one-pot synthesis of highly substituted pyridin-2(1H)-ones via the Vilsmeier-Haack reaction of 1-acetyl,1-carbamoyl cyclopropanes.

Organic letters·2007
Same author

Effects of Andrographitis Paniculata extracts on the expression of CD40 in endothelial cells.

Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban·2007
Same author

Theoretical and experimental investigations on the size of alginate microspheres prepared by dropping and spraying.

Journal of microencapsulation·2007
Same author

Beneficial effects of losartan on vascular injury induced by advanced glycosylation end products and their receptors in spontaneous hypertension rats.

Molecular and cellular biochemistry·2007
Same journal

Analysis of strength degradation of coal and rock masses and stability of mined areas under long term immersion environment.

PloS one·2026
Same journal

Biogenic Silver-Selenium nanocomposite with anticancer activity and potent efficacy against vancomycin-resistant Staphylococcus aureus.

PloS one·2026
Same journal

Preparation and physicochemical characterization of a biodegradable chitosan/carboxymethyl cellulose hydrogel synthesized in NaOH/urea medium.

PloS one·2026
Same journal

Action-guilt, survivor-guilt, and depression in combat-related PTSD.

PloS one·2026
Same journal

Explainable machine learning for predicting activities of daily living at discharge in stroke patients: A retrospective study using SHAP interpretability.

PloS one·2026
Same journal

Deep learning based two-way feature depiction model for brain tumor detection.

PloS one·2026
See all related articles

Related Experiment Video

Updated: May 18, 2026

The Insect Galleria mellonella as a Powerful Infection Model to Investigate Bacterial Pathogenesis
13:00

The Insect Galleria mellonella as a Powerful Infection Model to Investigate Bacterial Pathogenesis

Published on: December 11, 2012

pH-controlled Bacillus thuringiensis Cry1Ac protoxin loading and release from polyelectrolyte microcapsules.

Wenhui Yang1, Kanglai He, Jie Zhang

  • 1School of Life Science, Beijing Institute of Technology, Beijing, China.

Plos One
|October 2, 2012
PubMed
Summary
This summary is machine-generated.

New microcapsules protect Bacillus thuringiensis (Bt) crystal proteins from environmental damage. This biopesticide formulation maintains insecticidal activity and offers a promising solution for pest control.

More Related Videos

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
08:27

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

Published on: January 7, 2019

Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells
09:14

Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells

Published on: August 20, 2018

Related Experiment Videos

Last Updated: May 18, 2026

The Insect Galleria mellonella as a Powerful Infection Model to Investigate Bacterial Pathogenesis
13:00

The Insect Galleria mellonella as a Powerful Infection Model to Investigate Bacterial Pathogenesis

Published on: December 11, 2012

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
08:27

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

Published on: January 7, 2019

Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells
09:14

Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells

Published on: August 20, 2018

Area of Science:

  • Biotechnology
  • Materials Science
  • Entomology

Background:

  • Bacillus thuringiensis (Bt) crystal proteins are effective biopesticides against insect larvae.
  • Environmental factors can degrade Bt proteins, limiting their efficacy.
  • Novel formulations are needed to enhance biopesticide stability and delivery.

Purpose of the Study:

  • To develop a microcapsule formulation for protecting Cry1Ac protoxins from Bacillus thuringiensis.
  • To ensure targeted release of the biopesticide in the insect midgut.
  • To evaluate the stability and insecticidal activity of the new formulation.

Main Methods:

  • Layer-by-layer self-assembly of poly(acrylic acid) (PAH) and poly(styrene sulfonate) (PSS) around a CaCO(3) core.
  • Encapsulation of fluorescein-labeled Cry1Ac protoxin (FITC-Cry1Ac) using low pH self-assembly.
  • Morphological analysis using Scanning Electron Microscopy (SEM) and encapsulation confirmation via Confocal Laser Scanning Microscopy (CLSM).
  • Bioassays with Asian corn borer to assess insecticidal activity and midgut-specific release under alkaline conditions.

Main Results:

  • Microcapsules were successfully fabricated and loaded with Cry1Ac protoxins, confirmed by SEM and CLSM.
  • The formulation demonstrated stability under heat and desiccation.
  • Encapsulated Cry1Ac protoxins exhibited insecticidal activity comparable to free protoxins against the Asian corn borer.
  • Protoxin release was triggered by alkaline conditions simulating the insect midgut.

Conclusions:

  • The developed microcapsule formulation effectively protects Bt Cry1Ac protoxins from environmental degradation.
  • The formulation ensures targeted release in the insect midgut, enhancing biopesticide efficiency.
  • This technology presents a promising approach for developing stable and effective biopesticides for field applications.