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Updated: May 18, 2026

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture
Published on: February 23, 2018
Short-coherence off-axis holographic phase microscopy of live cell dynamics
Stefan Witte1, Andrius Plauşka, Margreet C Ridder
1These authors contributed equally to this work ; LaserLaB, VU University Amsterdam, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands.
Abstract:
We demonstrate a single-shot holographic phase microscope that combines short-coherence laser pulses with an off-axis geometry. By introducing a controlled pulse front tilt, ultrashort pulses are made to interfere over a large field-of-view without loss of fringe contrast. With this microscope, quantitative phase images of live cells can be recorded in a full-field geometry without moving parts. We perform phase imaging of HEK293 cells, to study the dynamics of cell volume regulation in response to an osmotic shock.
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