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Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Related Experiment Video

Updated: May 18, 2026

Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos
08:43

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Published on: June 6, 2016

Genetically Engineered Mice by Pronuclear DNA microinjection.

Janet L Demayo1, Jie Wang, Dongcai Liang

  • 1Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas.

Current Protocols in Mouse Biology
|October 2, 2012
PubMed
Summary
This summary is machine-generated.

Generating transgenic mice via DNA microinjection allows for studying gene expression, development, and disease. This powerful method introduces foreign DNA into embryos for phenotypic analysis in vivo.

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Last Updated: May 18, 2026

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Published on: June 6, 2016

Generation of Genetically Modified Mice through the Microinjection of Oocytes
10:19

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Published on: June 15, 2017

Mouse Genome Engineering Using Designer Nucleases
12:04

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Published on: April 2, 2014

Area of Science:

  • * Molecular Biology
  • * Developmental Biology
  • * Genetics

Background:

  • * Transgenic mice are crucial models for studying gene function and disease mechanisms.
  • * DNA microinjection is a primary technique for creating these genetically modified organisms.
  • * Understanding the process is key to advancing biological research.

Purpose of the Study:

  • * To outline the essential steps in generating transgenic mice using DNA microinjection.
  • * To highlight the significance of this technology in biological and biomedical research.
  • * To emphasize the need for efficient protocols to manage costs.

Main Methods:

  • * Preparation of transgene DNA constructs.
  • * Collection of one-cell stage fertilized mouse embryos.
  • * Microinjection of transgene DNA into pronuclei of embryos.
  • * Transfer of manipulated embryos into surrogate dams.
  • * Screening of offspring for transgene integration.

Main Results:

  • * Successful generation of transgenic mice carrying foreign DNA.
  • * Phenotypic analysis of genetically modified mice under physiological conditions.
  • * Establishment of invaluable research models for disease and development studies.

Conclusions:

  • * DNA microinjection remains a fundamental technique for creating transgenic mice.
  • * Efficient execution of the generation process is vital for cost-effectiveness.
  • * Transgenic mice are indispensable resources for advancing scientific understanding.