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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Updated: May 18, 2026

Sampling Human Indigenous Saliva Peptidome Using a Lollipop-Like Ultrafiltration Probe: Simplify and Enhance Peptide Detection for Clinical Mass Spectrometry

Sampling Human Indigenous Saliva Peptidome Using a Lollipop-Like Ultrafiltration Probe: Simplify and Enhance Peptide Detection for Clinical Mass Spectrometry

Published on: August 7, 2012

Top-down platform for deciphering the human salivary proteome.

Massimo Castagnola¹, Tiziana Cabras, Federica Iavarone

¹Istituto di Biochimica e Biochimica Clinica, Facoltà di Medicina, Università Cattolica, Largo Francesco Vito 1, Rome, Italy. massimo.castagnola@icrm.cnr.it

The Journal of Maternal-Fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians

|October 3, 2012

Summary

This study presents a catalog of 256 human saliva protein and peptide masses identified using top-down proteomic analysis. This resource aids researchers studying the human salivary proteome and other bodily fluids.

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Last Updated: May 18, 2026

Sampling Human Indigenous Saliva Peptidome Using a Lollipop-Like Ultrafiltration Probe: Simplify and Enhance Peptide Detection for Clinical Mass Spectrometry

Sampling Human Indigenous Saliva Peptidome Using a Lollipop-Like Ultrafiltration Probe: Simplify and Enhance Peptide Detection for Clinical Mass Spectrometry

Published on: August 7, 2012

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Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles

Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles

Published on: October 10, 2013

Area of Science:

Proteomics
Analytical Chemistry
Biochemistry

Background:

Proteomic strategies include bottom-up (digested) and top-down (intact) analyses.
Bottom-up offers high throughput but may lose PTM and structural data.
Top-down analysis reveals protein maturation variations and allows label-free quantification.

Purpose of the Study:

To create a reference repertoire of naturally occurring proteins and peptides in human saliva.
To detail masses, elution times, PTMs, and variations for identified salivary components.
To support the application of top-down RP-HPLC-ESI-MS in human salivary proteome research.

Main Methods:

Analysis of the acidic soluble fraction of human whole saliva.
Utilized reversed-phase high-performance liquid chromatography electrospray ionization mass spectrometry (RP-HPLC-ESI-MS).
Employed top-down proteomic strategies for intact protein and peptide analysis.

Main Results:

A repertoire of 256 masses from human saliva proteins and peptides was generated.
233 of these masses were definitively identified.
23 masses are pending definitive characterization.
Data includes average/mono-isotopic masses, RP-HPLC elution times, PTMs, and origin.

Conclusions:

The study provides a valuable reference for top-down RP-HPLC-ESI-MS analysis of the human salivary proteome.
The catalog aids in understanding quali-quantitative variations in physiological and pathological states.
This resource is applicable to the study of other human bodily fluids.