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Microarray analysis of microRNA expression in cutaneous squamous cell carcinoma
Michael Sand1, Marina Skrygan, Dimitrios Georgas
1Department of Dermatology, Venereology and Allergology, Ruhr-University Bochum, 44791 Bochum, Germany. michael.sand@ruhr-uni-bochum.de
Journal of Dermatological Science
|October 3, 2012
Summary
This study identified specific microRNAs (miRNAs) that are differentially expressed in cutaneous squamous cell carcinoma (cSCC). These findings offer potential biomarkers for cSCC molecular pathogenesis.
Area of Science:
- Molecular Biology
- Genetics
- Oncology
Background:
- MicroRNAs (miRNAs) are epigenetic regulators of gene expression.
- miRNA dysregulation is implicated in various cancers.
- Limited data exists on miRNA expression in cutaneous squamous cell carcinoma (cSCC).
Purpose of the Study:
- To identify differentially expressed miRNAs in cSCC.
- To establish miRNA expression profiles for cSCC.
Main Methods:
- Microarray analysis of miRNA expression profiles from cSCC tumor biopsies and adjacent healthy skin.
- Validation of microarray results using quantitative real-time polymerase chain reaction (qRT-PCR).
Main Results:
- Thirteen miRNAs were up-regulated and eighteen were down-regulated in cSCC at p ≤ 0.05.
- Three miRNAs (hsa-miR-135b, hsa-miR-424, hsa-miR-766) were significantly up-regulated and six (hsa-miR-30a*, hsa-miR-378, hsa-miR-145, hsa-miR-140-3p, hsa-miR-30a, hsa-miR-26a) were significantly down-regulated at p ≤ 0.01.
Conclusions:
- This study identified differentially expressed miRNAs in cSCC.
- These miRNAs are potential candidates for understanding cSCC molecular pathogenesis.
- Further validation and functional analysis of these miRNAs are warranted.
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