Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of Hydrophilic Brush Length and Hydrophobic Chain on Biodistribution of Polymethacrylate-Based Statistical Copolymers.

Biomacromolecules·2026
Same author

Catalyst-Controlled Regioselectivity Switching in the Bromolactonization of (<i>Z</i>)-Stilbenecarboxylic Acids.

Organic letters·2026
Same author

Correction: Conditional generation of free radicals by selective activation of alkoxyamines: towards more effective and less toxic targeting of brain tumors.

Chemical science·2026
Same author

Mitofusins are required for specialized mitochondrial morphology and function of rod photoreceptor cells.

Frontiers in cell and developmental biology·2026
Same author

Assessment of Solvation Models for Quantum Chemistry Calculations through A-Value Determination and Conformational Equilibrium Analysis of Difluorocyclohexanes as Benchmark Systems.

Journal of computational chemistry·2026
Same author

Neudesin modulates IGF1 and insulin signaling pathways by regulating the surface expression of IGF1R through a conserved WPE motif.

The FEBS journal·2026

Related Experiment Video

Updated: May 18, 2026

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
11:56

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

Published on: April 11, 2014

Rapid and convenient detection of ascorbic acid using a fluorescent nitroxide switch.

Yuta Matsuoka1, Mayumi Yamato, Toshihide Yamasaki

  • 1Department of Bio-functional Science, Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka 812-8582, Japan.

Free Radical Biology & Medicine
|October 3, 2012
PubMed
Summary

A new Naph-DiPy nitroxide probe rapidly detects ascorbic acid (vitamin C) by enhancing fluorescence. This method is specific and useful for diagnosing diseases related to ascorbic acid levels.

More Related Videos

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
08:23

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
08:25

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

Published on: December 25, 2016

Related Experiment Videos

Last Updated: May 18, 2026

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
11:56

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

Published on: April 11, 2014

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
08:23

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
08:25

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

Published on: December 25, 2016

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Medical Diagnostics

Background:

  • Ascorbic acid (vitamin C) is a vital reductant and antioxidant, crucial for collagen synthesis and preventing oxidative stress.
  • Deficiency in ascorbic acid leads to scurvy due to impaired collagen hydroxylation.
  • Accurate and rapid detection of ascorbic acid is important for disease diagnosis.

Purpose of the Study:

  • To develop a novel, sensitive, and specific fluorophore-nitroxide probe for detecting ascorbic acid.
  • To evaluate the probe's utility in biological samples for diagnostic purposes.

Main Methods:

  • Development of the Naph-DiPy nitroxide probe, which couples a fluorophore with a nitroxide moiety.
  • Utilizing the principle of fluorescence quenching and enhancement upon reaction with ascorbic acid.
  • Testing the probe's specificity against other reductants and reactive oxygen species.
  • Measuring ascorbic acid levels in rat plasma using the developed probe.

Main Results:

  • The Naph-DiPy nitroxide probe showed rapid reaction and significant fluorescence enhancement specifically with ascorbic acid.
  • The probe did not respond to other common reductants or reactive oxygen species, indicating high specificity.
  • Successful measurement of ascorbic acid in plasma samples from rats fed an ascorbic acid-deficient diet was demonstrated.

Conclusions:

  • The novel Naph-DiPy nitroxide probe offers a sensitive and convenient method for ascorbic acid detection.
  • This probe holds potential as a valuable diagnostic tool for diseases associated with ascorbic acid levels.