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Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
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Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...

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Related Experiment Video

Updated: May 18, 2026

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
08:41

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

SPP tomography: a simple wide-field nanoscope.

L Grave de Peralta1, C J Regan, A A Bernussi

  • 1Department of Physics, Texas Tech University, Lubbock, Texas, USA. luis.grave-de-peralta@ttu.edu

Scanning
|October 3, 2012
PubMed
Summary

Surface plasmon polariton (SPP) tomography enables wide-field optical nanoimaging of features smaller than the diffraction limit. This technique resolves nanoscale objects with unprecedented detail, offering a new tool for subwavelength imaging.

Keywords:
imagingmetal coatingsopticsphysical sciencessurface analysistomography

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Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography
08:51

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

Published on: May 27, 2008

Related Experiment Videos

Last Updated: May 18, 2026

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
08:41

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography
08:51

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

Published on: May 27, 2008

Area of Science:

  • Optics and Photonics
  • Nanotechnology
  • Materials Science

Background:

  • Optical microscopy faces limitations in resolving nanoscale features due to diffraction.
  • Subwavelength resolution techniques are crucial for advancing fields like nanotechnology and materials science.
  • Surface plasmon polaritons (SPPs) offer potential for overcoming diffraction limits in imaging.

Purpose of the Study:

  • To explore the wide-field optical nanoimaging capabilities of surface plasmon polariton (SPP) tomography.
  • To demonstrate the resolution limits of SPP tomography for nanoscale features.
  • To discuss the advantages of SPP tomography compared to other subwavelength resolution techniques.

Main Methods:

  • Utilizing surface plasmon polariton (SPP) tomography for nanoimaging.
  • Exciting SPPs via near-field fluorescence and coupling to the far-field through leakage radiation.
  • Forming wide-field surface emission (SE) images directly on a microscope camera.

Main Results:

  • Observed nanofeatures with lateral dimensions smaller than λ/20 in SE images of plasmonic crystals.
  • Resolved two single objects with a center-to-center separation of 200 nm and edge-to-edge separation of λ/7.
  • Demonstrated wide-field imaging not limited by out-of-plane diffraction.

Conclusions:

  • SPP tomography provides a powerful wide-field optical nanoimaging technique.
  • The method achieves subwavelength resolution, surpassing conventional optical limits.
  • SPP tomography offers unique imaging processing capabilities through analog tomography.