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Related Concept Videos

Cell Migration01:09

Cell Migration

Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.

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Related Experiment Video

Updated: May 18, 2026

From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia
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From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia

Published on: October 19, 2014

Live-cell migration and adhesion turnover assays.

J Lacoste1, K Young, Claire M Brown

  • 1Department of Biology, Cell Imaging and Analysis Network, McGill University, Montreal, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|October 3, 2012
PubMed
Summary

Live-cell fluorescence microscopy requires careful monitoring of cell health and minimizing light exposure. This guide details methods for cell viability assessment, light reduction strategies, and quantitative imaging techniques for cell migration and dynamics.

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From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia
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Published on: October 19, 2014

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Area of Science:

  • Cell biology
  • Microscopy
  • Biophysics

Background:

  • Fluorescence microscopy is a powerful tool for live-cell imaging but can harm specimens.
  • Monitoring cell viability and health is crucial during live-cell imaging.
  • Minimizing light exposure is essential for successful live-cell experiments.

Purpose of the Study:

  • To provide a comprehensive guide to live-cell fluorescence microscopy.
  • To discuss methods for monitoring cell viability and health.
  • To present strategies for minimizing light exposure and quantitative imaging.

Main Methods:

  • Discusses cell viability and health monitoring techniques.
  • Summarizes strategies for minimizing light exposure, including microscope optimization and detector sensitivity.
  • Details sample preparation and measurement methods for cell migration and focal adhesion turnover.
  • Explains image correction techniques for quantitative fluorescence imaging.

Main Results:

  • Presents various fluorescence microscopy techniques, evaluating light delivery methods and suitability for live specimens.
  • Describes methods for quantitative imaging, including background correction and uniformity adjustments.
  • Offers guidance on measuring cell migration rates and focal adhesion dynamics.

Conclusions:

  • This chapter serves as a valuable resource for scientists performing live-cell fluorescence microscopy.
  • It is particularly beneficial for researchers focused on quantitative imaging, cell migration studies, and focal adhesion dynamics.