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Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

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Related Experiment Video

Updated: May 18, 2026

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
09:38

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Published on: November 26, 2013

MALDI imaging mass spectrometry for direct tissue analysis.

Stephan Meding1, Axel Walch

  • 1Institute of Pathology, Helmholtz Zentrum Munchen, German Research Center for Environmental Health, Neuherberg, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|October 3, 2012
PubMed
Summary

Matrix-Assisted Laser Desorption/Ionization (MALDI) imaging mass spectrometry analyzes molecular distribution in tissues. This method correlates molecular patterns with tissue morphology, enabling precise localization of molecules to their cellular origins.

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Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)
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Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)

Published on: March 24, 2016

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Last Updated: May 18, 2026

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
09:38

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Published on: November 26, 2013

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)
10:47

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)

Published on: March 24, 2016

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Molecular Biology

Background:

  • Tissue analysis requires methods that preserve spatial information.
  • Understanding molecular distribution is crucial for disease diagnosis and research.
  • Current techniques may have limitations in preserving tissue morphology or analyzing diverse molecules.

Purpose of the Study:

  • To present a protocol for Matrix-Assisted Laser Desorption/Ionization (MALDI) imaging mass spectrometry.
  • To enable the analysis of proteins within tissue sections.
  • To demonstrate the capability of analyzing both native and fixed tissues.

Main Methods:

  • Utilizing MALDI imaging mass spectrometry for molecular analysis.
  • Applying the protocol to native and alcohol-fixed, paraffin-embedded tissue samples.
  • Correlating molecular data with tissue morphology.

Main Results:

  • MALDI imaging mass spectrometry successfully analyzed the spatial distribution of proteins in tissues.
  • The method maintained the morphological integrity of the tissue samples.
  • Specific molecules were accurately assigned to their cellular origins.

Conclusions:

  • MALDI imaging mass spectrometry is a powerful tool for in situ molecular analysis of tissues.
  • This technique allows for the direct correlation of molecular patterns with tissue architecture.
  • The presented protocol facilitates protein analysis in diverse tissue preparation states.