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Updated: May 18, 2026

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
Published on: September 21, 2017
Design of nuclease-resistant fork-like small interfering RNA (fsiRNA)
Elena L Chernolovskaya1, Marina A Zenkova
1Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russia. elena_ch@niboch.nsc.ru
Abstract:
Small interfering RNAs (siRNAs) are potent inducers of RNA interference--the conservative cellular process of posttranscriptional gene silencing. The silencing activity of siRNAs depends on the thermodynamic asymmetry of the siRNA duplex. Here, we describe the design of chemically modified fork-like siRNA (fsiRNAs) containing mismatches at the 3'-end region of the sense strand and 2'-O-methyl modifications in nuclease-sensitive sites, capable of silencing of thermodynamically unfavorable targets.
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