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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.

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Related Experiment Video

Updated: May 18, 2026

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
08:07

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

A versatile protein microarray platform enabling antibody profiling against denatured proteins.

Jie Wang1, Kristi Barker, Jason Steel

  • 1Virginia G. Piper Center for Personalized Diagnostics, Biodesign Institute, Arizona State University, Tempe, AZ 85287-6401, USA.

Proteomics. Clinical Applications
|October 3, 2012
PubMed
Summary
This summary is machine-generated.

A novel protein microarray platform presents both natural and denatured proteins for improved antibody biomarker discovery. This versatile tool enhances detection and offers new diagnostic potential for diseases.

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Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
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06:01

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Published on: January 7, 2019

Area of Science:

  • Biotechnology
  • Immunology
  • Proteomics

Background:

  • Protein microarrays are crucial for antibody biomarker discovery.
  • Current platforms face limitations in presenting diverse protein conformations.
  • Optimizing plasma screening is essential for sensitive autoantibody detection.

Purpose of the Study:

  • To develop a versatile protein microarray platform capable of displaying both native and denatured proteins.
  • To optimize plasma screening protocols for enhanced antibody detection.
  • To enable discovery of novel antibody biomarkers for disease diagnosis.

Main Methods:

  • Developed a covalent capture protein microarray using HaloTag technology.
  • Utilized in vitro transcription translation (IVTT) with HeLa cell lysate for enhanced protein yield.
  • Incorporated Escherichia coli lysates in blocking buffer to minimize nonspecific background.
  • Probed microarrays with plasma samples, comparing autoantibody responses with and without denaturing conditions.

Main Results:

  • The covalent attachment chemistry successfully maintained protein integrity under denaturing conditions.
  • Escherichia coli lysate blocking significantly reduced background noise.
  • HeLa cell lysate-based IVTT boosted antibody signal detection.
  • Denatured protein arrays revealed distinct autoantibody profiles compared to native protein arrays.

Conclusions:

  • The developed protein microarray platform supports both native and denatured protein display.
  • This versatility expands the search for disease-specific autoantibodies.
  • The platform broadens the scope of potential biomarkers and may lead to improved clinical diagnostics.