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Updated: May 18, 2026
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Single-Cell Sorting of Immunophenotyped Mesenchymal Stem Cells from Human Exfoliated Deciduous Teeth
Published on: November 10, 2023
Human mesenchymal stem cells: from immunophenotyping by flow cytometry to clinical applications
Arthur A Nery1, Isis C Nascimento, Talita Glaser
1Departamento de Bioquímica, Universidade de São Paulo, São Paulo, Brazil.
Summary
Regenerative medicine, particularly using mesenchymal stem cells (MSCs), offers new hope for tissue repair. These cells, identified by specific markers, are being explored in clinical trials for various diseases.
Area of Science:
- Regenerative Medicine
- Stem Cell Biology
- Immunology
Background:
- Regenerative medicine is poised to significantly advance tissue repair for degenerative diseases and injuries.
- Stem cell implantation, combined with growth factors, can improve patient quality of life.
- Mesenchymal stem cells (MSCs) are a promising therapeutic option due to ease of isolation.
Purpose of the Study:
- To highlight the role of mesenchymal stem cells (MSCs) in regenerative medicine.
- To define the key surface markers for MSC identification and purification.
- To summarize the therapeutic potential and clinical applications of MSC transplantation.
Main Methods:
- Cell isolation and purification from bone marrow and adipose tissues.
- Immunophenotyping using flow cytometry to identify MSCs based on surface marker expression (CD73+, CD90+, CD105+, CD34-, CD45-, HLA-DR-).
- Evaluation of MSC differentiation potential and clinical trial outcomes.
Main Results:
- MSCs can be reliably identified by a specific immunophenotype.
- Purified MSCs can be expanded and predifferentiated for therapeutic use.
- Clinical trials show promising results for treating bone, heart, and neurodegenerative diseases.
Conclusions:
- Mesenchymal stem cells represent a viable tool for tissue regeneration and repair.
- Autologous transplantation of MSCs minimizes rejection risks.
- Ongoing clinical trials demonstrate the broad therapeutic potential of MSCs in modern medicine.
