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Crystallization and preliminary X-ray diffraction analysis of alanine racemase from Pseudomonas putida YZ-26
Junlin Liu1, Lei Feng, Yawei Shi
1Institute of Biotechnology, Key Laboratory of Chemical Biology and Molecular Engineering of the Ministry of Education, Shanxi University, Taiyuan, People's Republic of China.
Abstract:
A recombinant form of alanine racemase (Alr) from Pseudomonas putida YZ-26 has been crystallized by the sitting-drop vapour diffusion method. X-ray diffraction data were collected to 2.4 Å resolution. The crystals belong to the space group C222(1), with unit-cell parameters a = 118.08, b = 141.86, c = 113.83 Å, and contain an Alr dimer in the asymmetric unit. The Matthews coefficient and the solvent content were calculated to be 2.8 Å(3) Da(-1) and approximately 50%, respectively.
