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Microsatellite markers for Amborella (Amborellaceae), a monotypic genus endemic to New Caledonia
Valérie Poncet1, Marie Couderc, Christine Tranchant-Dubreuil
1Institut de Recherche pour le Développement (IRD), Unité Mixte de Recherche-Diversité Adaptation et Développement des plantes (UMR DIADE), BP 64501, 34394 Montpellier Cedex 5, France. alerie.poncet@ird.fr
Premise Of The Study:
Informative markers are required for assessing the diversity of Amborella trichopoda, the only species of its order, endemic to New Caledonia and considered to be the sister species to all flowering plants. Therefore, expressed sequence tag (EST)-based microsatellite markers were developed. •
Methods And Results:
Fifty-five microsatellite loci were characterized in 14896 putative unigenes, which were generated by assembling A. trichopoda ESTs from the public sequence database. Seventeen markers revealed polymorphism in 80 adult shrubs from three populations. The number of alleles per locus ranged from two to 12, with a total of 132 alleles scored. The mean expected heterozygosity per population ranged from 0.336 to 0.567. •
Conclusions:
These markers offer an appropriate amount of variation to investigate genetic diversity structure, gene flow, and other conservation issues.
