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Published on: February 22, 2010
Isolation and characterization of 13 novel polymorphic microsatellite markers for Pinus koraiensis (Pinaceae)
Jing-Hua Yu1, Chang-Mei Chen, Zhong-Hua Tang
1State Engineering Laboratory of Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, Heilongjiang, People's Republic of China.
Premise Of The Study:
Thirteen polymorphic microsatellite markers were developed to investigate the genetic diversity and population structure of Pinus koraiensis. •
Methods And Results:
Using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) method with three specific PCR primers for screening the positive clones, 13 loci were found to be polymorphic in 78 individuals of P. koraiensis. Across all of the P. koraiensis samples, the number of alleles per locus ranged from two to 11. •
Conclusions:
These polymorphic markers will be useful for conservation genetics studies of this species and to inform the development of effective P. koraiensis conservation programs.
